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Updated: May 5, 2026

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

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通过Tau-STED成像和3D定量定位的Lytic颗粒标记物的免疫突触的分析

Emilia Scharrig1, Maria L Sanmillan1, Claudio G Giraudo1

  • 1Department of Microbiology and Immunology- Sydney Kimmel Medical College- Thomas Jefferson University, Philadelphia, PA, United States.

Methods in cell biology
|February 7, 2025
PubMed
概括

研究人员开发了一种新方法,使用Tau-STED显微镜对质颗粒进行定量分析. 这种技术克服了传统显微镜研究免疫细胞相互作用的局限性.

关键词:
细胞毒性T淋巴细胞免疫学的突触.刺激性排放耗尽 (STimulated emission depletion) 是指一种刺激性的排放耗尽.超高分辨率显微镜的使用方法

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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