组织剪切作为平面极性对齐的暗示在发展中的Drosophila翅膀中的平面极性
1School of Biosciences, University of Sheffield, Firth Court, Sheffield, UK.
Nature communications
|February 7, 2025
概括
机械力引导上皮细胞的极性. 高度组织应激影响Frizzled蛋白质的稳定性,并促进细胞流动,在发育的组织中建立近距离 (PD) 极性对齐.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 表皮平面细胞极性 (PCP) 的建立依赖于解释定向组织线索.
- 组织形态发生过程中的机械力越来越被认为是关键的定向线索.
- 连接机械力与PCP调节的机制仍然不太清楚.
研究的目的:
- 研究机械力,特别是组织应力如何调节平面极性.
- 阐明组织应激异构性在PCP建立和维护中的作用.
- 了解底层的分子机制力诱导的极性对齐.
主要方法:
- 使用了Drosophila幼翅膀模型系统.
- 分析了组织应力和应力异构性对平面极性的影响.
- 量化平面极性蛋白 (Frizzled) 在细胞结点的积累和稳定性.
- 研究了细胞流动动力学和细胞与细胞接触时的蛋白质循环.
主要成果:
- 证实组织应力促进近距离 (PD) 平面极性对齐.
- 证明高组织应激异质性降低了Frizzled蛋白质的积累和稳定性.
- 观察到在高应力异质性下细胞流梯度的增加,细胞行间的速度差异.
- 表明细胞流动促进了与流动方向平行的结点的核心蛋白质周转.
结论:
- 细胞流动的梯度对于建立和维持PD导向的极性至关重要.
- 与细胞流动相关的剪切力可能会解离跨膜复合体,影响蛋白质周转.
- 机械力和由此产生的细胞流动动力学是表皮平面极性的关键调节者.
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