来自高血压妊娠患者的血会诱导内皮功能障碍,即使在动脉动脉保护性剪切应力下
Sarah Viana-Mattioli1,2, Miriam Helena Fonseca-Alaniz2, Iguaracy Pinheiro-de-Sousa2,3
1Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Distrito Rubião Júnior, Botucatu, São Paulo, SP, Brazil.
Scientific reports
|February 7, 2025
概括
在不同的血液流动条件下,子宫前和妊娠高血压对内皮细胞的影响不同. 孕前血诱导了促炎和抗氧化反应,而孕期高血压血则在层状剪切应力下促进了细胞增殖.
科学领域:
- 心血管生物学 心血管生物学
- 生殖医学 生殖医学
- 分子遗传学 分子遗传学
背景情况:
- 孕前 (PE) 是与内皮功能障碍 (ED) 相关的重大孕产妇健康问题.
- 以前的研究还没有充分探讨血流模式,特别是层状 (LSS) 和振荡式 (OSS) 剪切应力在与PE相关的ED中的作用.
- 在缺氧下释放的胎盘因素与引发ED有关.
研究的目的:
- 研究来自健康孕妇 (HP),妊娠高血压 (GH) 和PE患者的血对人类冠状动脉内皮细胞 (HCAEC) 的影响.
- 在暴露于不同类型的血时,在LSS和OSS条件下分析HCAECs的基因表达特征.
- 阐明剪切应激如何影响内皮细胞对高血压妊娠状态的反应.
主要方法:
- 用HP,GH和PE受试者的血来化HCAEC.
- 被处理的HCAEC暴露在受控层切削应力 (LSS) 和振荡切削应力 (OSS) 条件下.
- 全球基因表达分析 (转录组分析) 以确定差异性基因表达模式.
主要成果:
- 层切削应力 (LSS) 在暴露于血的内皮细胞中诱导了一种独特的转录特征.
- 振荡剪切应力 (OSS) 导致类似的转录组,无论血来源如何.
- 在LSS下,GH血导致了增殖性内皮细胞形状,而PE血与HP血相比,诱导了促炎和抗氧化特征.
结论:
- 剪切应激模式显著调节内皮细胞基因表达,以响应高血压复杂的怀孕中的血.
- PE和GH都可能导致内皮功能障碍,即使在动脉保护性LSS条件下.
- 与LSS下的GH血相比,PE衍生血对内皮细胞转录组表现得更为明显.
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