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Updated: May 29, 2025

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

对长非编码基因表达的分析及其与血管内皮细胞中的蛋白质编码基因的相互作用,以及在 keloids 中的基因相互作用

Yunxiang Luo¹, Zi Ye², Yi Li²

¹Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-sen University, No. 58 Zhongshan Road 2, Guangzhou, 510080, China.

European journal of medical research

|February 7, 2025

概括

这项研究表明,血管内皮细胞 (VEC) 中的长非编码RNA (lncRNA) 和信使RNA (mRNA) 在 keloid 中表达差异,这表明它们通过表观遗传调节在 keloid 发育中的作用.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
皮肤病学皮肤病学

背景情况:

质体是由于细胞外基质沉积过多而造成的异常痕.
基洛因形成的基础分子机制,特别是非编码RNA的作用,仍然不完全理解.

研究的目的:

研究从 keloid 组织的血管内皮细胞 (VEC) 中传递 RNA (mRNA) 和长非编码 RNA (lncRNA) 的差异表达和相互作用.
为了确定潜在的调节网络,涉及到 keloid 病原体.

主要方法:

使用R语言对基因表达综合 (GEO) 微阵列数据 (GSE121618) 的重新分析.
对mRNAs和lncRNAs的差异表达分析.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
构建 lncRNA-mRNA 相互作用网络.
使用磁性排序VEC和定量实时PCR (qRT-PCR) 的验证.

主要成果:

在 keloid 和正常皮肤组织之间观察到 305 mRNA 和 98 lncRNA 的显著差异表达.
确定了关键的lncRNA-mRNA相互作用,包括LINC01546-RASAL3/COL13A1 (上调) 和LOC101929787-PRKAA2/KRT71/SSTR1 (下调).
qRT-PCR验证没有显示出统计学上显著的差异,这表明验证步骤的潜在限制或相互作用的复杂性.

结论:

生物信息学分析表明,VEC内部的lncRNA-mRNA相互作用在表观基因组调节 keloid 发育和进展中起着至关重要的作用.
这些发现为化体的分子基础和潜在的治疗点提供了新的见解.

关键词:

生物信息学是一种生物信息学.在 GEO 数据库中.凯洛伊德 (Keloid) 是一种类型的细胞.这是一个LncRNARNA.血管内皮细胞血管内皮细胞

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