利用番茄衍生的小细胞外囊泡作为癌症治疗药物输送系统
Kartik Kumar Sarwareddy1, Anula Divyash Singh1, Sreekanth Patnam1
1Apollo Hospitals Educational and Research Foundation (AHERF), Apollo Hospitals, Hyderabad, India.
Future science OA
|February 7, 2025
概括
番茄水果衍生的细胞外囊泡 (TsEVs) 提供了一种可持续的方法来提供,增强其抗癌作用. 这种方法克服了传统方法的局限性,显示了结肠癌治疗的前景.
科学领域:
- 生物技术是生物技术.
- 纳米医学是一种纳米医学.
- 在瘤学瘤学.
背景情况:
- 来自哺乳动物细胞的小细胞外囊 (sEVs) 面临着药物输送产量和成本方面的挑战.
- 酸是一种具有抗癌潜力的维生素D类似物,但需要有效的输送系统.
- 可持续和可扩展的药物输送平台对于推进癌症疗法至关重要.
研究的目的:
- 为了研究番茄水果衍生小细胞外囊泡 (TsEVs) 作为一种新的药物输送平台,.
- 评估通过TSEVs传递的酸盐对结肠癌细胞的抗癌疗效.
- 建立TsEVs作为一个具有成本效益和可扩展的替代哺乳动物sEVs.
主要方法:
- 使用离心和超过来分离TSEV,其特点是DLS,TEM和生物化学分析.
- 使用超声波优化了对TSEV加醇的加载,使用HPLC测量效率.
- 结肠癌细胞系 (HCT116,HT29) 用TSEV-calcitriol进行治疗,以评估生存能力,殖民地形成,迁移,ROS和亡.
主要成果:
- 尺寸为30-200纳米的TSEV显示出高效的酸封装 (47.3%通过超声波).
- TsEV-calcitriol显著降低了结肠癌细胞活力 (IC50:在48小时内2.07微克/毫升),并抑制了殖民地形成和迁移.
- 治疗提高了活性氧物种 (ROS) 水平,并增加了Bax/Bcl-2比率,表明诱导了亡.
结论:
- TsEVs代表了一个可行的和可持续的平台来提供酸,增强其抗癌性质.
- 这种植物衍生的外体组方法为癌症治疗提供了哺乳动物sEV的有希望的替代方案.
- 可以有效地利用TSEV来输送其他抗癌药物,扩大其治疗潜力.
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