周围单细胞子集在怀孕期间发生变化,在卵细胞捐赠怀孕中,怀孕并发症与先兆子

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Updated: May 29, 2025

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Published on: May 21, 2015

Xuezi Tian^1,2, Jia Li¹, Kim van Bentem²

¹Department of Immunology, Leiden University Medical Center, Leiden, Netherlands.

Scandinavian journal of immunology

|February 7, 2025

概括

卵子捐赠怀孕有更高的子宫前风险. 孕产妇单细胞在子宫前时表现出改变的CD38和免疫标志物表达,这可能表明炎症和血管功能障碍.

科学领域:

免疫学免疫学免疫学
生殖医学生殖医学
产科产科产科产科产科

背景情况:

卵子捐赠 (OD) 怀孕面临更高的胎儿与母亲不相容性和子宫前 (PE) 风险.
孕妇单细胞对于在怀孕期间耐受异性胎儿至关重要.

研究的目的:

分析健康和PE OD怀孕中的单细胞表型.
调查与怀孕结果相关的单细胞子集组成和表面标记物表达.

主要方法:

从各种妊娠阶段的健康 (n=10) 和PE (n=5) OD怀孕中收集母亲的外周血液.
计算的胎儿 - 母亲人类白细胞抗原 (HLA) 不匹配.
使用35色抗体面板用于奥罗拉光谱流细胞计,以分析单细胞子集.

主要成果:

在健康的OD怀孕中,中介单细胞上的CD38表达随着怀孕增加.
与健康对照组相比,PE OD 怀孕的单细胞子组上的 CD38 表达显著更高.
免疫抑制受体 (CD85j,CD85d) 和单细胞招募分子 (CCR2,CD91) 在PE OD怀孕中被上调,即使具有高HLA不匹配.

结论:

在健康的OD怀孕中,CD38的升级表明了第三个月的炎症状态.
在PE OD怀孕中,单细胞上的炎症和免疫调节标志物增加.
在PE中CCR2和CD91的升高可能表明单细胞化学反应和血管功能障碍.

关键词:

单细胞 (Monocytes) 是一种单细胞.捐赠卵子细胞捐赠孕产妇产前炎症 (pre-eclampsia) 是一个疾病.

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