相关实验视频

Updated: May 29, 2025

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

在口腔癌中,PPIA通过CD147增强细胞生长和转移

En-Chi Liao¹, Ching-Hsuan Law¹, Hsin-Yi Chen¹

¹Department of Medical Science and Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan.

Archives of biochemistry and biophysics

|February 8, 2025

概括

二烯异构酶A (PPIA) 通过增强细胞入侵和迁移,促进口腔癌转移. 降低PPIA水平或向它可能为口腔癌提供新的治疗策略.

科学领域:

在瘤学瘤学.
生物化学生化学
分子生物学分子生物学

背景情况:

口腔癌的死亡率显著且在不断上升.
分泌的蛋白质在生理过程中至关重要,并具有作为癌症生物标志物的潜力.
鉴定口腔癌转移的生物标志物对于有效的治疗策略至关重要.

研究的目的:

通过使用秘密基因分析,识别与口腔癌有关的分泌蛋白质.
为了研究乙烯基异构酶A (PPIA) 在口腔癌转移中的作用.
探索PPIA作为潜在的诊断和治疗目标.

主要方法:

秘密分子分析以确定候选蛋白质.
RNA干扰,以评估PPIA对细胞入侵和迁移的影响.
重组PPIA治疗以评估其对癌细胞行为的直接影响.
免疫组织化学分析以比较PPIA在瘤和正常组织中的表达.
分析下游信号通路 (ERK1/2,p38 MAPK) 和与CD147.7的相互作用.

主要成果:

秘密基因分析确定PPIA是口腔癌中关键的分泌蛋白质.
减少PPIA表达和分泌显著抑制了口腔癌细胞的入侵和迁移.
再组合PPIA治疗显著增加了口腔癌细胞的增殖,迁移和入侵.
与正常组织相比,在口腔瘤组织中观察到更高的PPIA表达.
发现PPIA可以激活ERK1/2和p38 MAPK信号通路,通过CD147.7促进转移.

结论:

PPIA在促进口腔癌细胞扩散和转移方面发挥着重要作用.
PPIA通过激活ERK1/2和p38 MAPK信号通路以及与CD147的相互作用而起作用.
PPIA是一个有前途的新型治疗标和口腔癌的预后标志物.

关键词:

CD147 是一个CD147 CD147 是一个CD147 转移转移转移转移口腔癌是一种口腔癌.这是一个PPIA,PPIA是PPIA.

更多相关视频

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Published on: July 27, 2022

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Cancer Cell Migration through Invadopodia

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...