在小鼠卵细胞中介成熟过程中,UBE2D3的功能
Guorui Zhang1, Na Zhang1,2, Bin Zhang1
1State Key Laboratory of Reproductive Medicine and Offspring Health, Changzhou Maternity and Child Health Care Hospital Changzhou Medical Center, Nanjing Medical University, Nanjing, China.
概括
对于小鼠卵细胞的介质分裂而言,E2无素结合酶UBE2D3至关重要. 它的失调有助于与年龄相关的生育率下降,这表明UBE2D3作为治疗点.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
- 遗传学 是一个遗传学.
背景情况:
- 乌比奎丁介导的蛋白质分解对于在变异过程中的细胞循环控制至关重要.
- 对于E2无素-结合酶在卵细胞半细胞分裂中的作用尚不清楚.
- 适当的染色体分离依赖于精确调节介质事件.
研究的目的:
- 为了研究E2在小鼠卵细胞中E2泛素结合酶的功能.
- 为了确定关键的E2酶参与介质忠实性.
- 探索UBE2D3在卵细胞衰老和生育能力中的作用.
主要方法:
- 确定UBE2D3是老鼠卵细胞中最丰富的E2酶.
- 在小鼠卵细胞中利用了UBE2D3的枯竭和过度表达.
- 评估了介质性进展,环素B1水平,动脉-微管附着物,螺旋组合检查点 (SAC) 功能和动脉化.
- 检查了老年小鼠的卵细胞,以将UBE2D3水平与介质缺陷相关联.
主要成果:
- UBE2D3对于介质分裂是必不可少的;耗尽会导致I元相停滞和B1循环蛋白积累.
- UBE2D3的过度表达导致了循环B1的减少,基内托霍尔-微管错误的附着,SAC功能障碍和动质积分.
- 老化卵细胞中UBE2D3的增加与中介性缺陷相关,通过UBE2D3敲击或Cyclin B1过度表达,部分可逆.
结论:
- UBE2D3-Cyclin B1轴对于保持卵细胞中介质忠实性至关重要.
- UBE2D3在与年龄相关的介质错误中起着重要作用.
- 准UBE2D3-Cyclin B1通路可能为改善老年女性卵细胞质量和生育能力提供治疗策略.
相关概念视频
02:02Meiosis II
42.7K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
42.7K
01:22Oogenesis
997
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
997
02:57Meiosis vs. Mitosis
52.4K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
52.4K