Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Structure01:23

RNA Structure

70.9K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
70.9K
Nucleic Acid Structure01:25

Nucleic Acid Structure

5.9K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
5.9K
RNA Stability01:53

RNA Stability

33.2K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.2K
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

3.7K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.7K
RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Experimental RNAi02:15

Experimental RNAi

6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Viral non-coding RNA structure annotation and API-based data retrieval with Rfam and R2DT.

bioRxiv : the preprint server for biology·2026
Same author

R3DCID: circular interaction diagrams for RNA/DNA.

RNA (New York, N.Y.)·2026
Same author

PDBe-SIFTS: an open-source tool for Structure Integration with Function, Taxonomy, and Sequences, featuring improved alignment, scoring scheme, and accelerated search.

bioRxiv : the preprint server for biology·2026
Same author

The regulation, function and disease relevance of cytoplasmic tRNAs.

Nature reviews. Molecular cell biology·2026
Same author

The diploid reference genome of a human embryonic stem cell line.

bioRxiv : the preprint server for biology·2026
Same author

The master molecule that built biology: How water shaped the chemistry of life.

Protein science : a publication of the Protein Society·2026

相关实验视频

Updated: May 29, 2025

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.3K

R2DT:用于可视化RNA二次结构的全面平台.

Holly McCann1,2,3, Caeden D Meade1,2, Loren Dean Williams1,2

  • 1NASA Center for Integration of the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, United States.

Nucleic acids research
|February 8, 2025
PubMed
概括

R2DT 2.0 增强了RNA二次结构可视化,提供了无模板和动画模式等新功能. 该软件允许交互式图形修改用于可复制RNA分析.

更多相关视频

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.0K
Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.5K

相关实验视频

Last Updated: May 29, 2025

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.3K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.0K
Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.5K

科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 分子生物学分子生物学

背景情况:

  • RNA二次 (2D) 结构可视化对于理解RNA功能至关重要.
  • 一致和可重复的RNA二维结构布局对于分析至关重要.
  • 现有的工具可能缺乏灵活性或高级可视化功能.

研究的目的:

  • 介绍R2DT 2.0,一个更新的软件包用于RNA 2D结构可视化.
  • 突出新功能,提高RNA可视化灵活性和互动性.
  • 将R2DT作为RNA二维结构分析的综合平台.

主要方法:

  • R2DT 2.0 结合了位置特定数据显示 (例如,SNP,SHAPE反应率).
  • 新模式包括无模板可视化,受约束的折叠和动画结构.
  • 支持通过手动输入或自然语言提示符进行交互式修改.
  • 包括性能增强和扩展模板库.

主要成果:

  • R2DT 2.0提供了具有增强功能的多功能RNA 2D结构可视化.
  • 用户可以通过交互式编辑生成新的模板和出版品质图像.
  • 该软件提供更快的性能和更广泛的适用性.
  • 将其集成到生物数据库中证明了它的实用性.

结论:

  • R2DT 2.0代表了RNA 2D结构可视化工具的重大进步.
  • 它的新功能和交互功能有助于更深入地了解RNA结构-功能关系.
  • 对于分子生物学和生物信息学研究人员来说,R2DT是一个有价值的,可访问的平台.