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使用网络节点操纵研究乳腺癌对小鼠微生物群殖民抗性的影响的协议
Ana Laura Cano-Argüelles1, Alejandra Wu-Chuang2, Lourdes Mateos-Hernandez2
1Parasitology Laboratory, Institute of Natural Resources and Agrobiology of Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
STAR protocols
|February 8, 2025
概括
本研究概述了一个网络分析协议,用于研究乳腺癌小鼠模型中的肠道微生物群落. 该方法详细介绍了瘤接种和16S rRNA数据处理,用于微生物共发生网络的构建.
科学领域:
- 微生物学 微生物学
- 生物信息学是一种生物信息学.
- 在瘤学瘤学.
背景情况:
- 肠道微生物群落在宿主健康和疾病中发挥着作用.
- 网络分析越来越多地用于理解复杂的生态相互作用.
- 肠道微生物组在乳腺癌发展中的作用是目前正在积极研究的一个领域.
研究的目的:
- 在乳腺癌小鼠模型中研究肠道微生物群落的详细方案.
- 为了使微生物共发生网络的构建使用16S rRNA数据.
- 为研究癌症中的宿主微生物相互作用提供一个框架.
主要方法:
- 建立乳腺癌的小鼠模型.
- 瘤细胞的生产和注射程序.
- 16S rRNA基因测序和数据处理.
- 基于丰度相关性构建微生物共发生网络.
主要成果:
- 该协议提供了一种可复制的方法来生成微生物社区数据.
- 同时发生的网络可以揭示肠道分类之间的复杂相互作用在乳腺癌的背景下.
- 这种方法有助于识别与疾病状态相关的特定微生物种群.
结论:
- 网络分析提供了一种强大的方法来剖析乳腺癌中的肠道微生物组变化.
- 该协议是研究主体微生物组与癌症相互作用的研究人员的宝贵资源.
- 该方法的进一步应用可以阐明微生物群落在癌症进展中的功能作用.
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