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Updated: May 29, 2025

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在口服纳斯塔暴露后,在小鼠肺和肝脏中形成DNA附加物

Morgan C Domanico¹, Nicole M Collette², Esther Ubick²

¹Center for Health and the Environment, University of California, Davis, Davis, CA 95616, United States.

Toxicological sciences : an official journal of the Society of Toxicology

|February 8, 2025

概括

纳夫他林暴露在小鼠肺部和肝脏中形成DNA附加物,持续长达72小时. 这表明了突变发生和逃避DNA修复机制的可能性.

科学领域:

环境健康环境健康
毒理学毒理学毒理学
分子生物学分子生物学

背景情况:

纳烯是一种常见的环境污染物和燃烧产物.
在动物中,慢性暴露会导致呼吸道瘤.
在体内内形成纳夫他林-DNA添加物的情况仍未得到证实.

研究的目的:

为了研究纳夫他-DNA添加物的体内形成和持久性.
在哺乳动物模型中确定纳夫他林暴露是否导致基因毒性.

主要方法:

通过口服给野生型C57BL/6小鼠 (50毫克/公斤) 的14C-纳фта.
使用加速器质谱法检测纳夫他林-DNA添加物.
分析不同时间点 (2-72小时) 肺和肝组织中的附加物.

主要成果:

在肺部和肝脏组织中检测到纳夫他林-DNA添加物.
随着时间的推移,adduct水平下降,但暴露后72小时仍然存在.
adducts 的持久性表明可能逃避 DNA 修复途径.

结论:

纳夫他林暴露导致DNA添加物在体内形成.
持久的引证表明存在突变发生的潜在风险.
进一步的研究是有必要的,以了解长期纳夫他暴露的影响.

关键词:

在DNA中,添加物是DNA的添加物.加速器质谱学质谱学加速器质谱学细胞染色体P450生物激活纳夫他烯纳夫他烯纳夫他

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