内毛细胞中的细胞外篮和听觉神经中的围神经网络:大鼠噪音引起的听力损失的变化
So Young Kim1, Jong Chan Jeon2, Bohyeon Park2
1Department of Anatomy and Cell Biology Seoul National University College of Medicine Seoul Republic of Korea; Sensory Organ Research Institute Seoul National University Medical Research Center Seoul Republic of Korea.
Neuroscience letters
|February 8, 2025
概括
丁硫酸盐蛋白质甘油 (CSPGs) 和和蛋白质甘油链接蛋白1 (HAPLN1) 存在于内毛细胞 (IHC) 细胞外篮中,并且随着噪声损伤而减少. 听觉神经在大腺细胞 (LGC) 上显示抑制性突触.
科学领域:
- 神经科学是一个神经科学.
- 审计系统研究 审计系统研究
- 细胞生物学 细胞生物学
背景情况:
- 耳内毛细胞 (IHCs) 的细胞外篮与调节突触合有关.
- 丁硫酸盐蛋白质甘油 (CSPGs) 和和蛋白质甘油链接蛋白1 (HAPLN1) 是这些细胞外结构的关键组成部分.
- 了解它们的表达和对受伤反应的变化对于听力健康至关重要.
研究的目的:
- 为了研究CSPGs (aggrecan,brevican,neurocan) 和HAPLN1在老鼠耳和听觉神经中的表达.
- 为了检查噪音诱导的耳损伤后CSPGs和HAPLN1表达的变化.
- 描述周围听觉神经细胞的周围神经网络 (PNN) 的分子组成部分.
主要方法:
- 利用免疫组织化学和免疫光学检测CSPGs (ACAN,BCAN,NCAN) 和HAPLN1在老鼠中的.
- 检查了听觉神经细胞的标记物,如帕瓦胺 (PV),GAD67,PSD95和GFAP.
- 采用现场杂交来评估布雷维坎 (BCAN) mRNA表达.
主要成果:
- 在IHC中检测到ACAN,BCAN和HAPLN1,在受噪声损伤的尾管中表达减少.
- 在螺旋状细胞 (SGC) 区域发现了ACAN和NCAN,但BCAN蛋白缺失.
- 听觉神经显示PV,GAD67和PSD95在大质细胞 (LGC) 周围的周围神经网络 (PNN) 中.
- 在听觉神经的SGC和质细胞中存在BCAN mRNA.
结论:
- IHC细胞外篮中的耳CSPGs和HAPLN1表达因噪音暴露而减少.
- 听觉神经在PV阳性LGC上具有PNN,表明抑制性突触.
- BCAN蛋白表达局限于IHC细胞外篮,而其mRNA在SGC中发现,表明差异调节.
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