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来自围产组织的介质干细胞通过PI3K/AKT激活促进糖尿病伤口愈合

Jiawei Huang¹, Qingwen Deng¹, Lai Ling Tsang¹

¹School of Biomedical Sciences, Faculty of Medicine; CUHK-GIBH CAS Joint Research Laboratory On Stem Cell and Regenerative Medicine; Key Laboratory for Regenerative Medicine of the Ministry of Education of China, The Chinese University of Hong Kong, Hong Kong SAR, China.

Stem cell research & therapy

|February 8, 2025

概括

来自围产组织的介质干细胞 (MSC),特别是人带 (hUCMSC) 和人胆 (hCVMSC),显著加快糖尿病伤口愈合. 这些MSC通过专门的水凝输送,促进组织再生和血管化.

科学领域:

再生医学是一种再生医学.
生物材料科学生物材料科学
伤口治愈研究研究伤口治愈研究

背景情况:

糖尿病足 (DFU) 是糖尿病的一个严重并发症,治愈结果不佳.
介质干细胞 (MSC) 疗法为DFU治疗提供了一个有前途的替代方案.
围产期MSCs (hUCMSCs,hCVMSCs,hDCMSCs) 在DFU模型中评估其治疗潜力.

研究的目的:

为了比较三种类型的周产期MSCs (hUCMSCs,hCVMSCs,hDCMSCs) 在促进糖尿病伤口愈合方面的疗效.
研究MSC介导的伤口修复的潜在机制.
评估新型水凝支架在DFU处理中适用于MSC输送的适用性.

主要方法:

在体外和体内研究比较hUCMSC,hCVMSC和hDCMSC的疗效.
对MSC分泌的蛋白质进行质谱分析.
将MSC纳入PEGDA/SA/Col-I水凝以评估伤口愈合.

主要成果:

与hDCMSC相比,hUCMSCs和hCVMSCs的伤口愈合能力更强.
MSCs增强了角质细胞的增殖,成熟和血管生成.
通过PI3K/AKT信号传递,MSC的分泌物促进了角质细胞和内皮细胞的功能.
PEGDA/SA/Col-I水凝支持MSC的活力和功能,加速伤口的关闭,改善组织的再生 in vivo.

结论:

周产期MSCs,特别是hUCMSCs和hCVMSCs,通过PI3K/AKT通路激活显著增强糖尿病伤口愈合.
通过支持MSCs,PEGDA/SA/Col-I水凝作为DFU治疗的有希望的支架.
特定的周产期MSC与优化的水凝配方相结合,代表了糖尿病伤口护理的重大进步.

关键词:

糖尿病伤口愈合的治疗方法水凝是一种水凝.多国中心的MSCs.在PI3K/AKT中使用.胎盘胎盘胎盘胎盘

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