从种子到溶液:通过高强度超声波,高压处理和pH转移来提高南瓜种子蛋白质分离物的技术功能和消化能力
1Department of Food Engineering, Igdir University, Iğdır 76000, Turkey.
Food chemistry
|February 9, 2025
概括
将pH值变化与高强度超声波 (HIUS) 结合起来,显著改善了南瓜种子蛋白分离物 (PSPI) 的功能. 这种新的方法比其他方法更有效地提高了溶解度,抗氧化活性和含量.
科学领域:
- 食品科学与技术 食品科学与技术
- 蛋白质化学 蛋白质化学
- 生物技术是生物技术.
背景情况:
- 黄瓜种子蛋白分离物 (PSPI) 具有作为功能性食品成分的潜力.
- 目前用于增强PSPI属性的方法的有效性往往有限.
- 优化PSPI功能对于扩展其应用至关重要.
研究的目的:
- 研究pH值变化与高强度超声波 (HIUS) 或高压处理 (HPP) 对PSPI的联合影响.
- 确定最佳的处理方法,以增强PSPI的结构和功能特性.
- 评估这些治疗对蛋白质溶解性,疏水性,抗氧化活性和含量的影响.
主要方法:
- PSPI接受了pH转移,HIUS,HPP以及它们的组合.
- 测量了关键的功能性质,包括可溶性,表面水性,自由硫含量,抗氧化活性和总含量.
- 使用热图分析分析结构变化.
主要成果:
- 值变化和HIUS的结合导致PSPI属性的最显著的改善.
- 这种治疗提高了蛋白质溶解性 (69.1%),表面疏水性 (251.1),自由硫含量 (5.88 μmol/g),抗氧化活性 (17.2%) 和总含量 (3.1 mg 甲基素/g).
- pH 变化 + HPP 也显示出改善,虽然不如 pH 变化 + HIUS. 个人治疗效果较差.
结论:
- 组合治疗,特别是pH转移+HIUS,在增强PSPI特性方面非常有效.
- 这些优化的PSPI显示了潜在的食品应用的功能改进.
- 这项研究强调了一种新且优越的蛋白质修饰方法.
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