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改善癌症光疗的ICG-ALA综合体

Mahshid Hashemkhani¹, Alphan Sennaroğlu², Havva Yağci Acar³

¹Koç University, Graduate School of Materials Science and Engineering, Rumelifeneri Yolu, Sariyer, 34450 Istanbul, Turkey; Université Paris Cité, Matiére et Systémes Complexes MSC, 45 rue des Saints Péres, 75006 Paris, France.

Journal of photochemistry and photobiology. B, Biology

|February 9, 2025

概括

这项研究介绍了一种新的组合疗法,使用5 - 氨基氨酸 (ALA) 和绿色 (ICG) 的静电结合,通过光动力学和光热疗法 (PDT / PTT) 加强癌症治疗. 结合ICG-ALA药物证明了指导光疗的卓越疗效和成像能力.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
癌症治疗癌症治疗

背景情况:

5 - 氨基氨酸 (ALA) 光动力疗法 (PDT) 和印ocyanine绿色 (ICG) 光热疗法 (PTT) 是已知的癌症治疗方法.
限制包括ALA的水友性和ICG在生理条件下的不稳定性.
结合PDT和PTT提供了一个有前途的治疗策略.

研究的目的:

通过对ALA和ICG进行静电结合,开发一种稳定,联合的PDT/PTT剂.
评估ICG-ALA复合物的生物相容性,细胞吸收和治疗疗效.
评估成像指导光疗的潜力.

主要方法:

ALA与ICG的静电结合形成了ICG-ALA复合体.
在体外评估SKBR3和MDA-MB-231癌细胞系中的生物相容性和细胞吸收.
与640/808nm光共辐射,以诱导同时进行PDT和PTT.
评估细胞活力,温度升高,氧化应激和卡斯帕酶3/7释放.
在近红外 (ICG) 和可见 (PpIX) 区域的体外成像.

主要成果:

与自由的ALA或ICG相比,ICG-ALA表现出极好的生物相容性和增强的细胞吸收.
在癌细胞中观察到显著的光毒性,在ICG-ALA的低度下,在同照射时.
在2.5μg ICG/mL-0.5mM ALA等效度下,几乎完全丧失了细胞活力.
观察到的温度升高和高氧化应激证实了PTT和PDT的出现.
通过ICG-ALA,可以在近红外和可见光谱区域对癌细胞进行可视化.

结论:

ALA和ICG的静电结合克服了个体剂的限制,创造了一个稳定有效的双作用光疗剂.
通过联合PDT和PTT机制,ICG-ALA在杀死癌细胞方面表现出卓越的疗效.
ICG-ALA的双模成像能力促进了成像指导的光疗,以改善临床结果.

关键词:

5 - 氨基氨基氨基氨基酸.结合性癌症治疗结合性癌症治疗印度氨酸绿色是绿色的光动力学疗法是一种光动力学疗法.光热疗法是一种光热疗法.第九类原氨酸原氨酸

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