相关实验视频

Updated: Jun 23, 2026

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Published on: June 5, 2012

EUCAST磁盘扩散方法用于口服无气生物的抗菌感应性测试

Anne Birkeholm Jensen¹, Ellen Frandsen Lau¹, Thomas Greve²

¹Department of Dentistry and Oral Health, Aarhus University, Aarhus, Denmark.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

|February 9, 2025

概括

需要对口服无气体进行标准化抗微生物敏感性测试 (AST). 欧卡斯特磁盘扩散方法需要延长Prevotella和Porphyromonas gingivalis的化时间,与Fusobacterium不同.

科学领域:

微生物学微生物学
临床诊断临床诊断临床诊断
传染性疾病传染性疾病

背景情况:

标准化抗微生物敏感性测试 (AST) 方法对于管理无氧细菌引起的口腔和口外感染至关重要.
目前的方法可能无法充分解决各种口腔无气生物的特定生长特征.

研究的目的:

评估最近公布的EUCAST磁盘扩散方法对快速生长的无氧生物和常见的口腔无氧细菌的适用性.
为了确定Prevotella spp., Porphyromonas gingivalis和Fusobacterium nucleatum复合物的精确AST的最佳潜伏时间.

主要方法:

对38种口服无氧菌株 (Prevotella spp.,P. gingivalis,F. nucleatum复合体) 进行了抗菌敏感性测试,使用了阿莫西西林和甲尼达片.
根据EUCAST指导方针,磁盘扩散板被无氧化化,抑制区在20小时和44小时后进行评估.

主要成果:

在所有测试的菌株中,推的基支持了生长.
对于Fusobacterium菌株来说,20小时的化时间就足够了.
在20小时内,Prevotella物种显示出不一致的生长,所有P. gingivalis菌株需要44小时才能进行可靠的区域评估.

结论:

欧卡斯特磁盘扩散方法适用于Fusobacterium nucleatum复合体,使用标准化.
对于精确的P. gingivalis和一些Prevotella物种的AST,需要长时间的潜伏期 (44小时).
对于缓慢生长的厌氧生物的长期化方案,需要进一步验证.

关键词:

抗生素耐药性抗生素耐药性口腔微生物口腔微生物牙周炎是指牙周炎的一种疾病.

更多相关视频

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Published on: October 15, 2014

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Published on: August 4, 2018

相关概念视频

Antimicrobial Effectiveness

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...