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在Yarrowia lipolytica中对烯的分泌和代谢工程

Liang Chai¹, Jiaxin Che¹, Xiaoxu Liu¹

¹State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, PR China.

Bioresource technology

|February 9, 2025

概括

研究人员利用ATP结合盒 (ABC) 载体和载体蛋白系统设计了Yarrowia lipolytica以分泌素. 这种方法实现了创纪录的3.43g/L细胞外素分泌,促进了微生物生产有价值的类素.

科学领域:

微生物生物技术微生物生物技术
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

烯是一种有价值的类化合物,在制药和化品中具有应用.
高效的微生物生产和分泌烯仍然具有挑战性,因为它具有不透膜的性质.
雅罗维亚脂质菌是微生物生产的强壮宿主,但需要工程来增强分泌.

研究的目的:

在Yarrowia lipolytica中开发一种有效的细胞外分泌素的系统.
为了识别和优化ATP结合盒 (ABC) 运输器和载体蛋白质用于石贩运.
建立一个微生物生产和分泌类的多功能平台.

主要方法:

squalene生物合成途径的过度表达.
对增强分泌的ATP结合盒 (ABC) 载体和氧结合蛋白 (OSH) 的选.
通过将OSH3结合域与分泌信号融合,设计一种载体蛋白介导的代谢物贩运系统.
信号的优化和食批量种植.

主要成果:

SNQ2和OSH3的组合显著改善了烯分泌.
工程化载体蛋白系统增加了素分泌量,达到144.67 mg/L (17倍改善).
该系统通过促进β-胡卜素分泌来证明了它的多功能性.
养批量种植实现了3.43g/L的细胞外分泌记录 (占总产量的27.2%).

结论:

在Y. lipolytica中建立了一个有效的分泌平台,用于在Y. lipolytica中产生膜不透的素.
载体蛋白介导的贩运系统为微生物生产类生物提供了一个有前途的战略.
这项研究为设计微生物宿主以分泌脂性化合物提供了宝贵的见解.

关键词:

代谢物分泌工程代谢物分泌工程这是一种OSH3蛋白质.斯克瓦林是一种质.雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.

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