通过工业重要的人工微生物生产酸和酸的进展
Na Wu1, Wenxin Wang2, Jianguo Zhu3
1College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, China.
Journal of biotechnology
|February 9, 2025
概括
微生物发酵为生产L-酸和酸等有机酸提供了一个可持续的替代方案. 代谢工程的进步正在改善产量,并减少工业应用的发酵时间.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 传统的有机酸生产方法 (植物提取,化学合成) 面临着环境污染,高成本和不可持续性等挑战.
- 微生物发酵是一种环保和经济高效的替代方案,利用多种碳来源生产有机酸.
- 目前微生物发酵的局限性包括低产量和延长的发酵周期,阻碍了工业的可扩展性.
研究的目的:
- 通过代谢工程来审查最近在增强有机酸生物合成方面的进展.
- 专注于通过微生物发酵改善L-酸 (L-MA) 和酸 (SA) 的生产.
- 讨论微生物有机酸生产领域的当前挑战和未来前景.
主要方法:
- 利用omics技术 (基因组学,转录组学) 来阐明有机酸生产菌株的代谢途径.
- 应用合成生物学工具和高通量查来改善菌株.
- 代谢工程策略,以优化生物合成途径的目标有机酸.
主要成果:
- 在理解和操纵微生物代谢途径以生产有机酸方面取得了重大进展.
- 代谢工程方法在提高L-酸和酸生物合成的产量和效率方面表现有前途.
- 先进的查和合成生物学技术的发展加速了优质微生物菌株的识别和工程.
结论:
- 由奥米克和合成生物学驱动的代谢工程对于克服微生物有机酸生产的局限性至关重要.
- 优化微生物发酵具有可持续和成本效益的有机酸工业供应的巨大潜力.
- 对代谢工程和工艺优化的进一步研究对于生物基有机酸的广泛工业应用至关重要.
相关概念视频
01:20Fates of Pyruvate
8.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.3K
01:35Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives
1.9K
Just like β-keto acids—which upon thermal decarboxylation form ketones—β-dicarboxylic acids undergo decarboxylation to generate monocarboxylic acids with the liberation of carbon dioxide.
1.9K
01:14Alkylation of β-Diester Enolates: Malonic Ester Synthesis
3.3K
Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.
3.3K
02:36The Citric Acid Cycle
150.7K
The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
150.7K
01:37The Citric Acid Cycle: Overview
15.9K
In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...
The citric...
15.9K
00:53Products of the Citric Acid Cycle
98.1K
The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
98.1K