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相关概念视频

RNA Splicing01:32

RNA Splicing

55.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.9K
Alternative RNA Splicing02:18

Alternative RNA Splicing

20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

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相关实验视频

Updated: May 28, 2025

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

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转录组范围的替代拼接和转录级差异表达分析死后的莱维体痴呆症大脑.

Thomas R Goddard1, Keeley J Brookes2, Kevin Morgan3

  • 1Institute of Mental Health, Mental Health and Clinical Neurosciences Academic Unit, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, UK.

Acta neuropsychiatrica
|February 9, 2025
PubMed
概括

勒维体痴呆症 (LBD) 显示出广泛的RNA拼接变化. 转录层次分析揭示了对于LBD病理学和潜在生物标志物至关重要的特定差异表达的转录.

关键词:
这就是GABRB3B3的原因.莱维体痴呆症 (Lewy体痴呆症) 是一种疾病.通过RNA测序进行RNA测序.在TEMEM18中,我们可以使用TEMEM.替代性拼接是一种替代性的拼接.

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科学领域:

  • 神经科学是一个神经科学.
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 莱维体痴呆症 (LBD) 是第二常见的痴呆症,分子病理学不明.
  • 在LBD大脑中,RNA拼接失调很普遍,这表明它在疾病机制中发挥了作用.

研究的目的:

  • 在死后的LBD大脑中进行第一个全转录组,转录级差异表达分析.
  • 通过检查RNA变化来确定LBD的新型治疗点和生物标志物.

主要方法:

  • 利用下一代RNA测序数据从前带带皮层 (ACC) 和背侧前额皮层 (DLPFC) 的LBD患者和对照.
  • 使用鱼进行转录量化,使用edgeR进行差异表达分析,使用DRIMseq进行替代拼接分析.

主要成果:

  • 在ACC中确定了74个差异表达的转录 (DET),在DLPFC中确定了96个.
  • 在ACC中发现了135个替代拼接基因,在LBD大脑的DLPFC中发现了98个.
  • 发现DET可能会影响DNA修复,细胞亡,神经可塑性和RNA调节.

结论:

  • 转录水平分析显示,基因水平研究错过了DET,为LBD分子病理学提供了更深入的见解.
  • 证实了广泛的替代拼接,没有慢性神经炎症的证据.
  • 特定的DET,特别是来自TMEM18,MICB,MPO和GABRB3,作为LBD生物标志物和治疗标显示出希望.