对临床肠球菌分离物产生的细菌素及其对包括VRE在内的肠球菌的抗菌活性进行了全面的分析
Ayumi Fujii1,2, Miki Kawada-Matsuo3,4, Mi Nguyen-Tra Le1
1Department of Oral and Maxillofacial Surgery, Hiroshima University Graduate School of Biomedical and Health Sciences, Kasumi1-2-3, Hiroshima City, Hiroshima, 734-8551, Japan.
Scientific reports
|February 9, 2025
概括
菌种类产生具有抗菌性质的细菌素. 这项研究分析了Enterococcus faecalis和Enterococcus faecium中的细菌素基因,揭示了它们对其他细菌的分布和活性,包括耐药菌株.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 已知Enterococcus物种可以产生具有抗菌活性的细菌素.
- 对Enterococcus菌株中的细菌素基因分布缺乏全面的分析.
研究的目的:
- 在Enterococcus faecalis和Enterococcus faecium菌株中识别细菌素基因.
- 根据这些菌株的细菌素基因配置文件,研究这些菌株的抗菌活性.
主要方法:
- 在80个E. faecalis和38个E. faecium菌株中对细菌素基因 (cytolysin,enterolysin A,BacL1,色素A,色素B,色素NKR-5-3B,细菌素T8,BacAS9) 的基因组鉴定.
- 对抗 E. faecalis 和 E. faecium 的抗菌活性进行表型检测,包括对菌素耐药的菌株.
主要成果:
- 在E. faecalis和E. faecium中确定了不同频率的特定细菌素基因.
- 在细菌素阳性菌株中观察到可变的抗菌活性,对其他肠球菌.
- 通过化素A,细菌素T8和BacAS9阳性菌株对抗万科米辛耐药菌株 (VRE) 的已证明活性.
结论:
- E. faecalis 和 E. faecium 菌株含有不同的细菌素基因.
- 细菌素的生产会影响抗菌谱,并可能影响肠球菌群体的组成.
- 某些产生细菌素的菌株显示出对抗VRE感染的潜力.
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