开发和优化一种与酸盐结合的乳酶生物催化剂及其在医院废水处理中的应用
Komla Alokpa1,2, Younes El-Yagoubi1,3, Hubert Cabana4,5
1Université de Sherbrooke Water Research Group (GREAUS), 2500 Boul. de L'Université, Sherbrooke, QC, J1K 2R1, Canada.
Environmental science and pollution research international
|February 9, 2025
概括
通过共价结合将乳酶酶固定在二氧化上,提高了其稳定性和效率,用于从医院废水中去除制药污染物. 这种优化的生物催化剂有效降解了乙氨基和其他微量有机污染物.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 生物催化剂是一种生物催化剂.
背景情况:
- 医院的废水含有类似药品的持续微量有机污染物 (TrOC),造成环境和健康风险.
- 乳糖酶显示出降解这些污染物的潜力,但在实际应用中通常需要稳定.
研究的目的:
- 开发和优化一个稳定的固定乳酶生物催化剂,以有效地从医院废水中去除TROC.
- 为了比较不同的固定化策略 (吸附与共价结合) 和交叉链接剂 (甲和素).
主要方法:
- 来自Trametes hirsuta的Laccase通过吸附和共价结合在氨基功能化上被固定.
- 使用Box-Behnken方法来优化固定过程.
- 描述包括SEM,BET表面积,BJH孔径大小分析和元素分析.
- 稳定性和催化活性被评估在医院的废水和针对特定的药物化合物.
主要成果:
- 使用谷氨 (GLA) 进行的共价固定,与吸附相比,产生了更高的特异活性,产量和稳定性.
- 优化的固定乳酶 (AFHMS-GLA-Lac) 显示出对pH值,温度和变质剂的增强稳定性.
- 生物催化剂有效地从真正的医院废水中去除了乙氨基 (83%) 和其他TROC,如美芬胺酸 (86%) 和碳胺 (62%).
- 乙氨基降解的催化参数 (Km,kcat,kcat/Km) 在自由和固定乳酸之间是相似的.
结论:
- 使用GLA对氨基功能化二氧化上酶的共价固定是一种有效的策略,用于创建强大的生物催化剂.
- 优化的固定乳糖酶显示出在医院废水中药物污染物的修复方面具有显著的潜力.
- 这项研究为开发先进的生物催化剂解决方案来应对复杂的废水处理挑战提供了宝贵的框架.
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