在模拟肠道模型中,多菌株乳酸细菌联盟的益生菌特征和生存情况
Muhammad Nadeem Khan1,2,3, Saeeda Bashir1, Afifa Afzal1
1Faculty of Biological Sciences, Department of Microbiology, Quaid-I-Azam University Islamabad, Islamabad, 45320, Pakistan.
Folia microbiologica
|February 9, 2025
概括
巴基斯坦大吉的乳酸细菌 (LAB) 显示出强大的益生菌潜力,能够在模拟的肠道条件下生存并表现出有益的活动. 一个多菌株联盟为功能性食品和治疗应用提供了增强的功能.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- 巴基斯坦传统发酵牛奶"大希"是富含乳酸细菌 (LAB) 的丰富来源.
- LAB 具有潜在的益生菌特性,对肠道健康和功能性食物的发展有帮助.
- 关于LAB的益生菌能力的研究有限.
研究的目的:
- 为了隔离和表征益生菌LAB从dahi.
- 在模拟的胃肠道条件下评估LAB生存率.
- 评估LAB菌株和多菌株联盟的安全性和益生菌潜力.
主要方法:
- 从37个达希样本中分离和表征143个LAB分离物.
- 在体外评估酸/胆耐受性,抗菌,降胆固醇和抗氧化作用.
- 对益生菌相关基因的基因分析和安全性测试 (抗生素敏感性,血液溶解/DNase活性).
主要成果:
- 62个LAB菌株被确定具有显著的酸和胆汁耐受性.
- 观察到高抗菌活性 (51-88%),胆固醇去除 (高达98%),和抗氧化活性 (高达76%).
- 基因组分析证实了益生菌基因;所有菌株都是安全的,对抗生素敏感,缺乏血液溶解/DNase活性.
结论:
- 达希实验室表现出显著的益生菌潜力,通过体外和基因组分析进行验证.
- 一个开发的多菌株联盟证明了优越的益生菌功能和生存.
- 这些发现支持dahi LAB在功能性食品和治疗中应用.
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