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LncRNA THUMPD3-AS1/microRNA-4465/KPNA2轴影响人类肝细胞癌细胞表型

Jiawei Wang¹, Chunzhong Qiao¹, Baoyang Luo¹

¹Department of Hepatobiliary and Pancreatic Surgery, Taizhou People's Hospital Affiliated to Nanjing Medical University, Taizhou 225300, Mainland, China.

Regenerative therapy

|February 10, 2025

概括

长非编码RNA THUMPD3-AS1促进肝细胞癌 (HCC) 的生长和迁移. 它通过抑制microRNA-4465来做到这一点,这导致KPNA2表达增加和上皮-介质细胞转换 (EMT).

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

肝细胞癌 (HCC) 是一个重要的全球健康问题.
长非编码RNA THUMPD3-AS1 (lncRNA THUMPD3-AS1) 与各种癌症有关.
在HCC进展中,lncRNA THUMPD3-AS1的特定作用需要进一步阐明.

研究的目的:

调查lncRNA THUMPD3-AS1在HCC中的作用.
在HCC.中探索lncRNA THUMPD3-AS1,microRNA-4465 (miR-4465) 和KPNA2之间的调控关系.
确定这种途径对HCC细胞表型和上皮层-介质细胞过渡 (EMT) 的影响.

主要方法:

对临床HCC标本进行分析,以量化lncRNA THUMPD3-AS1,KPNA2,miR-4465和EMT标记物的水平.
在体外实验中,使用sh-THUMPD3-AS1或miR-4465模仿的HCC细胞转染.
对分子相互作用和对HCC细胞行为的功能后果的验证.

主要成果:

在HCC组织中,lncRNA THUMPD3-AS1和KPNA2过度表达,而miR-4465则降低.
lncRNA THUMPD3-AS1直接针对miR-4465,从而导致KPNA2的上调.
沉默lncRNA THUMPD3-AS1或恢复miR-4465抑制了HCC细胞的增殖,迁移和EMT.

结论:

lncRNA THUMPD3-AS1促进HCC细胞的生长和迁移.
这种瘤效应是由lncRNA THUMPD3-AS1/miR-4465/KPNA2轴介导的.
lncRNA THUMPD3-AS1通过竞争性抑制miR-4465和上调KPNA2.2来诱导HC细胞中的EMT.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.肝细胞癌是肝细胞癌.在 KPNA2 中设置 KPNA2.这就是LncRNA THUMPD3-AS1 的原因.微型RNA-446565是什么意思移民移民移民

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