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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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相关实验视频

Updated: May 28, 2025

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
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lncRNA局部化和特征解释性分析.

Jing Li1,2, Ying Ju3, Quan Zou4

  • 1Department of Microbiology, University of Hong Kong, Hong Kong, China.

Molecular therapy. Nucleic acids
|February 10, 2025
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概括
此摘要是机器生成的。

我们开发了LncDNN,这是一种新的计算模型,可以准确地预测长非编码RNAs (lncRNAs) 在细胞核和细胞核中的亚细胞局部,从而提高我们对它们的生物学作用的理解.

关键词:
MT: 生物信息学 生物信息学在SHAP分析中,我们分析了SHAP.功能选择 功能选择在 lncRNAs 中.机器学习是机器学习.核的核心是核的核.核塑料是核塑料中的一种.亚细胞局部化的局部化

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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 生物分子的细胞下定位,包括长非编码RNA (lncRNAs),对于理解细胞功能和调节机制至关重要.
  • lncRNAs在细胞过程中扮演着不同的角色,它们在细胞核和核质等部分的特定位置提供了关于它们的功能和疾病相关性的见解.

研究的目的:

  • 开发和验证一个计算模型,LncDNN,用于准确预测核细胞和核质中的lncRNA定位.
  • 通过分析影响模型的关键特征来提高lncRNA本地化预测的可解释性.

主要方法:

  • 开发LncDNN模型,使用三个不同的编码方案.
  • 应用沙普利增量解释 (SHAP) 来进行特征分析和选择,以确保模型的可解释性.
  • 对现有模型进行 lncRNA 局部化预测的比较性性能评估.

主要成果:

  • 与其他现有模型相比,LncDNN在预测lncRNA定位方面表现出卓越的准确性.
  • 使用SHAP的特征分析提供了有关lncRNA定位的关键序列和结构决定因素的见解.
  • 该模型的有效性得到了验证,用于识别核和核质中的lncRNA定位.

结论:

  • LncDNN是一种非常准确和可解释的工具,用于预测lncRNA亚细胞定位.
  • 该模型有助于更深入地了解 lncRNA 功能及其在生物过程和疾病中的参与.
  • 这些发现有助于推进IncRNA生物学研究及其对健康和疾病的影响.