相关实验视频

Updated: May 28, 2025

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

在急性缺血性中风患者中循环PIWI相互作用RNA

Salman M Toor¹, Eman K Aldous¹, Aijaz Parray²

¹College of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar.

Non-coding RNA research

|February 10, 2025

概括

与PIWI相互作用的RNAs (piRNAs) 是中风的新生物标志物. 这项研究在急性缺血性中风 (AIS) 和短暂缺血性发作 (TIA) 患者中确定了10种差异表达的piRNA,提供了新的诊断和预后潜力.

科学领域:

生物化学生化学
遗传学是一个遗传学.
神经学神经学

背景情况:

在全球范围内,中风是导致死亡和残疾的首要原因,往往是短暂性缺血性发作 (TIA) 之前.
循环非编码RNAs (ncRNAs) 显示出作为中风生物标志物的前景.
之前在中风诊断或预后方面没有研究PIWI相互作用RNA (piRNAs).

研究的目的:

探索循环piRNAs作为急性缺血性中风 (AIS) 和TIA的潜在生物标志物.
为了确定在AIS和TIA患者中差异表达的特定piRNAs.

主要方法:

在AIS和TIA患者的血清样本上使用RNA测序进行全面的循环piRNA分析.
将队列分层成发现和交叉验证数据集,以进行可靠的piRNA识别.
在AIS,TIA和健康对照组之间比较piRNA表达水平.

主要成果:

在AIS和TIA患者中确定了一个由10个不同调节的piRNA组成的小组.
八个piRNAs (hsa-piR-28272, -piR-32972, -piR-28247, -piR-24553, -piR-24552, -piR-28275, -piR-28707, -piR-32882) 在AIS中进行了上调调.
两个piRNAs (hsa-piR-23058, -piR-23136) 在AIS下调,所有10个都与健康对照相比表达差异.

结论:

循环piRnome变化与AIS和TIA患者的缺血性脑损伤有关.
这些发现表明piRNAs是潜在的中风诊断和预后生物标志物.
对piRNA的进一步研究可能会提供新的策略来降低与中风相关的死亡率和发病率.

关键词:

生物标志物生物标志物缺血性缺血性缺血性缺血性没有编码的RNA.皮维维皮维维皮维维一次性中风,中风.这就是为什么piRNARNA.

更多相关视频

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Published on: February 12, 2016

Author Spotlight: Establishing a Reliable Distal MCA Occlusion Model in Mice for Stroke Research

Author Spotlight: Establishing a Reliable Distal MCA Occlusion Model in Mice for Stroke Research

Published on: December 15, 2023

相关概念视频

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...