相关实验视频

Updated: May 28, 2025

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

隔离,特征和新型病毒性沙门氏菌菌体vB-SeS-01的脂质体载封装

Yuhang Luo¹, Jacques Mahillon², Lin Sun¹

¹College of Life Sciences, South-Central Minzu University, Wuhan, China.

Frontiers in microbiology

|February 10, 2025

概括

一种新的菌体,vB-SeS-01,有效地准沙门氏菌,提供一种潜在的抗生素替代品. 脂质体配方增强了其稳定性和传递性,显示了细胞内抗菌应用的前景.

科学领域:

微生物学微生物学
细菌治疗疗法是一种细菌治疗.
药物输送系统药物输送系统

背景情况:

沙门氏菌是一种通过食物传播的病原体,它表现出可选的细胞内寄生虫行为,使抗生素治疗复杂化.
菌体为抗生素提供了可行的替代品,用于控制细胞内和可选细胞内细菌.

研究的目的:

为了隔离和表征一种针对Salmonella enterica的新型细菌菌体.
开发和优化菌体携带脂肪体配方,以提高稳定性和传递性.
评估菌体及其脂质体配方对细胞内沙门氏菌的抗菌疗效.

主要方法:

分离和表征菌体vB-SeS-01.1.
使用膜水化和挤出方法开发了九种携带菌体的脂质体配方.
菌体溶解能力,可复制性,稳定性 (pH,温度) 和脂质体封装效率,大小和稳定性的评估.
评估细胞内抗菌作用和减少炎症因素.

主要成果:

菌体vB-SeS-01对9/24种Salmonella enterica菌株表现出强烈的溶解,具有高可复制性 (爆裂大小为111±15) 和广泛的稳定性 (pH为4.013.0,温度为480°C).
一种优化的脂质体配方 (PC:Chol:T80:SA = 9:1:2:0.5) 实现了高封装度 (80%),稳定性,并保持了菌体标位 (10^11 PFU/mL).
菌体vB-SeS-01及其脂质体配方都表现出细胞内抗菌活性和减少炎症因子转录.

结论:

菌体vB-SeS-01是控制沙门氏菌感染的有希望的候选者.
脂质体封装显著提高了菌体的稳定性和潜在的治疗应用的输送.
菌体载脂质体对沙门氏菌具有有效的细胞内抗菌作用,减少宿主炎症反应.

关键词:

格恩西岛的病毒已经消失了.沙门氏菌 (Salmonella enterica) 已经成为一种常见的疾病.细胞内抗菌作用细胞内抗菌作用脂质体组是什么? 脂质体组是什么?菌体菌体是一种菌体.

更多相关视频

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

相关概念视频

Lytic Cycle of Bacteriophages

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...