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在全血中基于GC/MS的硫化物离子的定量分析,使用乙烯硫尼尔化物作为衍生试剂

Ryosuke Shiraki^1,2, Shin Ogawa³, Kengo Wakigawa³

¹Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan. s.ryosuke.8975@kyudai.jp.

Forensic toxicology

|February 10, 2025

概括

一种新方法简化了血液中的硫化物离子分析,使用乙烯硫尼尔化物 (ESF) 进行气色谱-质谱 (GC/MS). 这种技术为法医和分析应用提供了可靠的量化.

科学领域:

法医化学法医化学
分析化学分析化学
生物化学生物化学

背景情况:

准确识别和定量生物样本中的硫化物离子对于法医目的至关重要.
传统的衍生试剂用于硫化物离子分析,使用气色谱-质谱 (GC/MS) 可能是复杂的,需要小心处理.
需要简化和可靠的方法来确定生物矩阵中的硫化物离子.

研究的目的:

评价乙烯硫尼尔化物 (ESF) 作为一种用于硫化离子分析的新型衍生试剂.
开发一种使用GC/MS的全血硫化物离子量化简化衍生协议.
评估基于ESF的法医应用方法的性能.

主要方法:

全血中的硫化物离子在简化的协议中使用乙烯硫尼尔化物 (ESF) 来衍生.
使用气体染色学-质谱学 (GC/MS) 分析了衍生样本.
评估了关键的分析参数,包括选择性,检测极限,线性,精度和准确性.

主要成果:

乙烯硫尼尔化物 (ESF) 在全血中对硫化离子具有较高的反应性和选择性,而不是干扰物质.
最优化的方法实现了硫化物离子0.01μg/ml的低检测极限.
在硫化物离子量化方面获得了优异的线性 (R2 = 0.9999),精度 (在±10%以内) 和准确性 (在±10%以内).

结论:

开发的GC/MS方法利用乙烯硫尼尔化物 (ESF) 提供了一种可靠和简化的方法,用于在全血中定量硫化离子.
该方法是法医调查和其他需要精确硫化物离子测量的分析领域的宝贵工具.
该研究强调了ESF作为一种有效的衍生剂在复杂生物样本中进行硫化离子分析的潜力.

关键词:

衍生产制衍生产制衍生产制乙烯硫尼尔化物乙烯硫尼尔化物气体染色学/质谱学硫化是一种硫化.硫化物离子硫化物离子

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