Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Reporter Genes02:11

Reporter Genes

11.2K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Covalent aptamers: agents with promising therapeutic and diagnostic potential.

RSC chemical biology·2025
Same author

Small-molecule control of CAR T cells.

Nature reviews. Chemistry·2025
Same author

Genetically Encoded Lysine Analogues with Differential Light Sensitivity for Activation of Protein Function.

ChemPhotoChem·2025
Same author

Inverted Molecular Beacons as Reaction-Based Hybridization Probes for Small-Molecule Activation by Nucleic Acid Inputs.

ACS chemical biology·2025
Same author

Rational Design of Stapled Covalent Peptide Modifiers of Oncoprotein E6 from Human Papillomavirus.

ACS chemical biology·2025
Same author

Conditional Control of Benzylguanine Reaction with the Self-Labeling SNAP-tag Protein.

Bioconjugate chemistry·2025

相关实验视频

Updated: May 28, 2025

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
08:20

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

7.9K

原子精度的光遗传学――通过遗传码扩展对蛋白质功能的光学控制的全面审查.

Maura Charette1, Carolyn Rosenblum1, Olivia Shade1

  • 1Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.

Chemical reviews
|February 10, 2025
PubMed
概括

研究人员使用遗传密码扩展结合光活性氨基酸,使细胞和动物对蛋白质活性进行精确的光学控制,用于功能研究.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 视觉遗传学 视觉遗传学

背景情况:

  • 条件控制蛋白活性对于理解细胞和生物的功能至关重要.
  • 光学方法可以在体内对生物过程提供精确的时空控制.
  • 遗传密码的扩展允许对非正规氨基酸进行特定位置的结合.

研究的目的:

  • 审查光和可光切换的非正规氨基酸 (ncAAs) 的应用,以光学控制蛋白质活性.
  • 突出使用遗传密码扩展来引入光活性残留物到蛋白质中的用途.
  • 证明光遗传工具在生物研究中的广泛适用性.

主要方法:

  • 基因代码扩展,用于结合光和可光切换的ncAAs.
  • 在关键蛋白质残留物中对光活性ncAAs的特定部位的整合.
  • 对各种蛋白质标和应用的审查.

主要成果:

  • 通过使用修改的ncAAs证明了蛋白质功能的成功光学激活/失活.
  • 展示了基于结构和机制数据的合并地点的合理选择.
  • 编制了一份广泛的蛋白质清单,可以进行光学控制.

更多相关视频

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.3K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

473

相关实验视频

Last Updated: May 28, 2025

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
08:20

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

7.9K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.3K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

473

结论:

  • 光活性ncAAs提供了一个强大的工具,可以实现对蛋白质功能的有条件的,光感应的控制.
  • 这种光遗传学方法可以详细研究蛋白质在复杂生物系统中的作用.
  • 该方法在各种蛋白质和研究领域广泛适用.