乙烯反应因子SlERF.D6通过转录因子SlDEAR2和SlTCP12部分促进成熟
Yao Chen1,2, Xin Wang1,3, Vincent Colantonio1,4
1Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853.
概括
在番茄中发现了一种新的乙烯反应因子 (ERF) 基因SlERF.D6. 它的破坏延迟了水果的成熟和胡卜素的积累,揭示了控制这一关键过程的新调节模块.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 发展水果发展水果发展
背景情况:
- 果实成熟对于种子的分散和营养价值至关重要,主要研究的基因调节剂在番茄中.
- 新出现的证据表明,局部组织在早期成熟基因活性中起着关键作用.
研究的目的:
- 识别和功能性地描述番茄中的新型成熟调节剂,重点关注活跃在局部组织中的基因.
- 阐明控制水果成熟的调控网络,包括特定组织的基因表达模式.
主要方法:
- 研究了番茄转录组数据在水果发育过程中,强调局部特定的基因表达.
- 利用CRISPR/Cas9基因编辑来创建SlERF.D6功能丧失突变体.
- 进行了转录组分析和促进体相互作用研究,以确定目标基因和调节途径.
主要成果:
- 鉴定和表征了SlERF.D6,一种参与成熟调节的乙烯反应因子 (ERF) 基因.
- SlERF.D6突变体表现出延迟的成熟开始和减少的胡卜素积累在周围肌和位置组织.
- 发现了涉及SlERF.D6,SlDEAR2和SlTCP12的调控级联,突出了组织特定的控制机制.
结论:
- SlERF.D6作为番茄果实成熟的重要调节剂,影响多种途径,包括胡卜素生物合成和乙烯信号传递.
- 一个新的三组分调节模块 (SlERF.D6-SlDEAR2-SlTCP12) 帮助复杂的网络控制水果成熟.
- 这项研究揭示了特定组织在成熟调节中的作用,并扩大了我们对水果发育遗传学的理解.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Transcription Elongation Factors
10.7K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
10.7K
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Diversity in Cell Signaling Responses
6.3K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...
Graded and Abrupt Responses
Some signaling systems generate...
6.3K


