具有不同纳米殖民地形态的细菌物种具有不同的流动依赖的殖民行为
Kelsey M Hallinen1, Steven P Bodine2, Howard A Stone3
1Department of Physics, Princeton University, Princeton, NJ 08544.
概括
液体流动显著影响细菌殖民. 金黄色葡萄球菌 (Staphylococcus aureus) 和菌 (Enterococcus faecalis) 使用不同的策略在高流量环境中壮成长,挑战了关于剪切应激对细菌感染影响的先前假设.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 流体动力学 流体动力学
背景情况:
- 在细菌环境中,流体流动至关重要,它会影响诸如感染之类的行为.
- 格拉姆阳性细菌如金黄色葡萄球菌 (Staphylococcus aureus) 和菌 (Enterococcus faecalis) 通过殖民心脏门引起内心炎,这在高剪切环境中是一种反直觉的行为.
研究的目的:
- 为了研究细菌对高剪率环境的偏好.
- 使用微流体系统量化流量条件对黄金葡萄球菌和菌菌殖民的影响.
主要方法:
- 开发一个微流体系统来控制和分析流体流动条件.
- 在不同剪切速率下对细菌殖民的量化.
- 实验和计算研究以阐明不同的殖民机制.
主要成果:
- 黄金葡萄球菌和菌菌都偏好在高流量环境中殖民,即使没有宿主因素.
- 金黄色葡萄球菌利用细胞群和剪切影响的分散信号.
- Enterococcus faecalis采用具有耐剪切机械性能的线性链.
结论:
- 细菌采用不同的机制,在高流量条件下实现优先殖民.
- 细菌的集体行为和纳米殖民地形态具有显著的,环境依赖的成本和收益.
- 了解流动对细菌集体相互作用的影响对于预测环境成功和感染动态至关重要.
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