在酵母中重演一只小鼠的遗传进化军备竞赛,揭示了SLXL1/SLX与SLY1/2在结合Spindlins方面竞争
Martin F Arlt1, Alyssa N Kruger1, Callie M Swanepoel1
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109.
概括
小鼠X和Y染色体基因家族,SLXL1/SLX和SLY,竞争结合斯宾林蛋白. 这种剂量依赖的竞争会影响精子健康和后代的性别比率.
科学领域:
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 家鼠的X和Y染色体具有快速演变的基因家族,SLXL1/SLX (X链接) 和SLY (Y链接).
- 这些基因家族与进化军备竞赛有关,这可能会扭曲后代的性别比率.
- 这些X和Y链接基因产物之间的精确竞争机制尚不清楚.
研究的目的:
- 阐明SLXL1/SLX和SLY蛋白质竞争的分子机制.
- 了解这种竞争如何影响精子健康和后代的性别比.
主要方法:
- 利用基于酵母的测试来研究蛋白质-蛋白质相互作用.
- 分析了蛋白质结合特异性和剂量依赖性.
- 检查了进化特征,如积极选择和基因增加/损失.
主要成果:
- SLXL1与SLY1和SLY2竞争,以与Spinlin SPIN1.1结合.
- SLX与SLY2竞争将其与斯宾林SSTY2.2结合起来.
- 竞争涉及SLXL1,SLX,SLY1和SLY2的N termini与Spindlins的第三个Tudor域结合,以及SLY1/SLY2形成二元体,表明多元体复杂的竞争.
结论:
- SLXL1/SLX和SLY蛋白质之间的竞争是基因家族特异的,并且取决于剂量.
- 这种竞争发生在平分体精子体中,影响X型与Y型精子的健康状况.
- 进化证据支持一种染色体间竞争模式,导致性别比率扭曲.
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