相关实验视频

Updated: May 5, 2026

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

化物通过IP3R1/MCU介导的线粒体过载通过MAMs诱导精子细胞亡

Xin Guo¹, Linyuan Wang¹, Jingyan Xuan¹

¹College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, Shanxi 030801, China.

Journal of hazardous materials

|February 10, 2025

概括

过度的化物暴露会破坏线粒体和内分泌网膜 (ER) 相互作用,从而损害精子质量. 这项研究表明,化物通过线粒体关联ER膜 (MAM) 干扰的转移,这表明化症的新治疗标.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

过度的化物暴露与精子质量降低和线粒体功能障碍有关.
线粒体-内质网膜 (ER) 相互作用对于精子细胞线粒体健康至关重要.
线粒体关联的ER膜 (MAMs) 介导这些器官之间的通信.

研究的目的:

为了研究MAMs在对化 (NaF) 暴露的反应中的分子机制.
探索 (Ca2+) 通过MAM转移在化物诱导的丸毒性中的作用.

主要方法:

小鼠被暴露于NaF (25-100毫克/升60天);GC-2spd细胞被NaF (24小时内1.5-2.5毫米) 治疗.
使用压力测试来评估线粒体功能.
分析了ER-线粒体接触,Ca2+水平,蛋白质表达 (IP3R1,GRP75,VDAC1,MCU,MFN1/2,VAPB,PTPIP51) 和细胞亡标志物 (细胞染色体C,Caspase-3).这些都被分析了.
干预包括Ru360和IP3R1siRNA,以评估它们对MAM和亡的影响.

主要成果:

化物暴露显著损害了线粒体呼吸和ATP的产生.
纳暴露增加了ER-线粒体接触,线粒体Ca2+水平和IP3R1,GRP75,VDAC1和MCU的表达.
化物降低了MFN1/2,VAPB,PTPIP51水平,并增加了亡标志物.
Ru360和IP3R1 siRNA治疗改善了线粒体功能障碍和减少了亡.

结论:

化物暴露通过IP3R1-GRP75-VDAC1-MCU轴通过MAMs促进Ca2+转移来破坏精子细胞功能.
抑制IP3R1或MCU可能代表一种潜在的治疗策略,用于控制化物诱导的毒性 (化症).

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.化物的化物.一个MAM就是MAM.线粒体中的Ca2+)精子细胞是精子细胞.

更多相关视频

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

相关概念视频

Fertilization

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...