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发现和功能性表征新的粉活性质多糖体单氧化酶的功能性表征

Nan Zhang¹, Junaid Haider¹, Maxine Yew²

¹State Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin 300308, PR China; Haihe Laboratory of Synthetic Biology, 21 West 15th Avenue, Tianjin Airport Economic Area, Tianjin 300308, PR China; National Technology Innovation Center of Synthetic Biology, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, PR China.

International journal of biological macromolecules

|February 10, 2025

概括

鉴定和描述了四种新型的粉活性Lytic多糖胺单氧酶 (LPMOs). FvAA13 LPMO显著增强了粉降解,显示了生物质糖化中的工业应用潜力.

科学领域:

生物化学生物化学
酶学是一种酶学.
生物技术是生物技术.

背景情况:

性多糖体单氧化酶 (LPMOs) 是在生物质糖化过程中分解反复性多糖体的关键酶.
粉活性LPMOs,特别是AA13家族成员,由于其独特的氧化裂解机制而引起人们的兴趣.

研究的目的:

为了识别和描述新的粉活性AA13LPMOs.
评估这些LPMOs在各种粉基板上的氧化活性.
评估高活性LPMO (FvAA13) 与其他粉降解酶的协同作用.

主要方法:

遗传学分析被用来确定潜在的LPMO候选人.
在Pichia pastoris中LPMOs的功能表达.
进行了酶定量测试,以测量粉素,粉素和玉米粉的氧化活性.
协同降解研究与α-amylase和glucoamylase结合进行.

主要成果:

成功识别和表达了四种新的粉活性AA13LPMOs.
这些LPMOs对粉素,粉素和玉米粉具有氧化活性,产生可溶性产品.
与之前报告的LPMOs相比,FvAA13显示出更高的氧化活性,产量通过H2O2.2.增强.
FvAA13显著促进了粉通过α-氨酶和葡萄氨酶的降解,特别是在与葡萄氨酶结合使用时,用于烯胺的水解.

结论:

发现了新的粉活性AA13LPMOs,扩大了这种酶类的已知多样性.
FvAA13代表了一种高效的LPMO用于粉氧化,并有可能使用过氧化进行优化.
将FvAA13添加到粉溶解酶尾酒中,可以大大提高粉的糖化,在工业生物技术中提供有前途的应用.

关键词:

辅助活动家族13 (AA13)性多糖体单氧化酶 (LPMOs) 是一种氧化活性活动的氧化作用.粉降解降解粉降解

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