相关实验视频

Updated: May 28, 2025

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

通过TaDRIM-seq同时对染色质关联RNA在目标DNA位置和RNA-RNA相互作用进行分析

Cheng Ding¹, Guoting Chen^1,2, Shiping Luan¹

¹National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.

Nature communications

|February 10, 2025

概括

研究人员开发了TaDRIM-seq,这是一种研究染色质相关RNA (caRNA) 和它们的相互作用的新方法. 这项技术揭示了大米中的昼间RNA-DNA和RNA-RNA网络,进步了我们对基因组调节的理解.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

细胞基因组产生各种RNA,其中许多与染色质相关.
染色体关联RNAs (caRNAs) 在基因调节和基因组组织中发挥作用.
询问caRNA及其相互作用仍然在技术上具有挑战性.

研究的目的:

介绍TaDRIM-seq,一种用于探测caRNAs和RNA-RNA相互作用的新技术.
为了使caRNAs在整个基因组区域和完整的核内进行并发分析.
为caRNA研究提供一种更有效,更少劳动密集的方法.

主要方法:

TaDRIM-seq利用了针对蛋白质G (PG) -Tn5的DNA元素.
现场近距离结合用于捕获RNA-DNA和RNA-RNA相互作用.
该方法在哺乳动物细胞和植物中得到验证,需要最小的细胞输入和实践时间.

主要成果:

TaDRIM-seq成功地在染色质环的DNA基区域识别了广泛的caRNAs.
这项研究揭示了RNA-DNA和RNA-RNA连接网络的日间变化.
在大米基因组中观察并表征了显著的caRNA网络.

结论:

TaDRIM-seq是研究caRNA及其全基因组相互作用的高效和多功能技术.
这些发现突显了植物RNA-DNA和RNA-RNA相互作用的动态性质.
这种方法可以更深入地了解caRNAs在基因调节和基因组组织中的作用.

更多相关视频

Chromatin Isolation by RNA Purification ChIRP

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...