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Updated: May 28, 2025

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
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A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

Published on: August 28, 2018

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一个强大的表观遗传编辑器,准人类PCSK9,以持续降低低密度脂蛋白胆固醇水平

Frederic Tremblay1, Qiang Xiong2,3, Shrijal S Shah2,3

  • 1Chroma Medicine, Boston, MA, USA. frederic.tremblay@chromamedicine.com.

Nature medicine
|February 10, 2025
PubMed
概括

表观遗传编辑提供了一种新的方法,可以在不改变DNA的情况下使疾病基因沉默. 这项研究表明,在动物中,PCSK9的沉默是持久的,可逆的,为治疗高胆固醇铺平了道路.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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