相关实验视频

Updated: May 8, 2025

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

使用人类诱导的多能干干细胞生成视网膜器官

Keith Theodore¹, Joel Alan Imventarza², Saleha Tahir¹

¹FM Kirby Center for Molecular Ophthalmology, Department of Ophthalmology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.

Methods in molecular biology (Clifton, N.J.)

|February 10, 2025

概括

来自干细胞的人类视网膜器官 (ROs) 模拟视网膜疾病并帮助基因疗法. 本研究详细介绍了一种生成这些3D结构并评估其形态学的方法.

科学领域:

生物医学研究的研究.
干细胞生物学干细胞生物学
眼科医生眼科眼科

背景情况:

人类诱导的多能干细胞 (hiPSCs) 对疾病建模有价值.
视网膜器官 (ROs) 模仿人类视网膜发育.
视网膜组织为研究视网膜疾病和基因疗法提供了一个平台.

研究的目的:

为从hiPSCs产生视网膜有机体提供详细的协议.
评估生成的RO的形态特征.

主要方法:

通过使用特定的协议,从hiPSC生成视网膜有机体.
对三维多细胞RO结构的形态评估.

主要成果:

成功生成复杂,多层的视网膜有机体.
人类视网膜发育和结构的回顾演示.

结论:

描述的方法有效地从hiPSCs产生视网膜有机体.
这些有机体作为视网膜研究和治疗开发的强大模型.

关键词:

人类诱导的多能干细胞 (hiPSCs)视网膜有机物视网膜有机物

更多相关视频

Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

Published on: April 12, 2021

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Published on: April 21, 2021

相关概念视频

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...