乌尔索西胆酸缓解了牛乳腺上皮细胞中棕酸诱导的亡
Md Rezwanul Habib1, Yukako Tokutake2, Shinichi Yonekura1,2
1Graduate School of Medicine, Science and Technology, Shinshu University, Nagano, Japan.
Animal science journal = Nihon chikusan Gakkaiho
|February 11, 2025
概括
Ursodeoxycholic 酸 (UDCA) 通过减少氧化和内质网膜压力,保护牛乳腺细胞免受棕酸 (PA) 的影响. UDCA增强了细胞活力并恢复了氧化还原平衡,有可能改善奶牛的牛奶产量.
科学领域:
- 细胞生物学 细胞生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 棕酸 (PA) 可以诱导牛乳腺上皮细胞 (bMECs) 的亡,影响奶牛的生产力.
- 奶牛的负能量平衡往往导致PA水平的增加,导致细胞应激.
研究的目的:
- 研究ursodeoxycholic acid (UDCA) 对MAC-T bMECs中PA诱导的亡的保护作用.
- 评估UDCA对细胞活力,氧化应激和内质网膜 (ER) 应激标志物的影响.
主要方法:
- 在暴露于PA之前,MAC-T细胞先用不同度的UDCA进行预处理.
- 评估了细胞活力,反应性氧物种 (ROS) 生产和ER压力标志物的表达 (GRP78,XBP1s,ATF4,CHOP).
- 评估谷氨 (GSH) 和谷氨二硫化物 (GSSG) 水平,以确定氧化还原平衡.
主要成果:
- UDCA显著改善了细胞活力,减少了PA.
- UDCA预处理降低了PA诱导的ER压力标志物和ROS产量.
- UDCA通过恢复GSH/GSSG比率来抵消PA诱导的氧化还原平衡的破坏.
结论:
- UDCA通过增强 redox 稳态和减少 ER 压力来缓解 bMEC 中的 PA 诱导的亡.
- 用UDCA补充奶牛料可以减轻细胞压力,并在负能量平衡期间改善乳生产.
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