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广谱抗菌抑制Vibrio parahaemolyticus 基于Lactobacillus paracasei A1发酵方式抑制Vibrio parahaemolyticus 基于Lactobacillus paracasei

Feng Guan^1,2, Chen Yu¹, Lu Yang¹

¹College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, China.

Foodborne pathogens and disease

|February 11, 2025

概括

来自Lactobacillus paracasei A1的抗菌有效地通过破坏细胞膜和生物膜来抑制Vibrio parahaemolyticus. 这些发现突显了它们作为食品添加剂的潜力,以控制食源性病原体并提高食品安全.

科学领域:

微生物学微生物学
食品科学食品科学食品科学
生物化学生物化学

背景情况:

食物传播的病原体,如Vibrio parahaemolyticus对公共健康构成重大风险.
抗微生物 (AMP) 对抗食物传播病原体有希望,但它们的机制各不相同.
来自Lactobacillus paracasei的AMP尚未完全描述它们对V. parahaemolyticus的有效性.

研究的目的:

调查来自Lactobacillus paracasei A1的AMP的抗菌谱和杀菌机制,以对抗Vibrio parahaemolyticus.
在原始提取物中识别功能性短,负责抗菌活性.
评估这些AMP作为食品添加剂的潜力,以控制食源性病原体.

主要方法:

对13种致病细菌进行抗微生物活性查.
对AMP对V. parahaemolyticus细胞膜完整性和生物膜形成的影响的评估.
最低抑制度 (MIC) 的确定.
液体染色学-双重质谱法 (LC-MS/MS) 用于的识别.

主要成果:

原始AMP提取物表现出广泛的抗菌特性,具有强烈的抑制V. parahaemolyticus.
AMPs破坏了V. parahaemolyticus细胞膜,抑制了初始粘附,生物膜形成和MICs的现有生物膜.
LC-MS/MS确定了四种短,它们对V. parahaemolyticus具有强大的细菌静止作用.
AMP通过抑制病原体的传播和增强抗微生物药物的有效性来降低食品安全风险.

结论:

来自Lactobacillus paracasei A1的抗菌有效地向细菌结构和生物膜.
这些AMP显示出作为天然食品添加剂的显著潜力,用于控制食品传播病原体,如V. parahaemolyticus.
进一步的研究可能会使用这些抗微生物来改善食品安全策略.

关键词:

牛乳杆菌帕拉凯西 (Lactobacillus paracasei) 是一种植物.这种病毒是Vibrio parahaemolyticus.抗微生物是一种抗微生物.生物膜是一种生物膜.

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