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使用AimPlex流式细胞计测试采样血细胞因子测试的最佳条件

Kaiwen Wang¹, Xueyu Zhang², Haiting Yang³

¹Department of Rheumatology, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, P.R. China.

Scandinavian journal of clinical and laboratory investigation

|February 11, 2025

概括

准确的细胞因子量化对于了解免疫调节和疾病诊断至关重要. 在-20°C保存长达24小时的凝血清样本可提供可靠的细胞因子水平,提高诊断准确度.

科学领域:

免疫学免疫学免疫学
生物化学生物化学
临床诊断临床诊断临床诊断

背景情况:

细胞因子量化对于理解免疫调节和诊断疾病至关重要.
检测方法和储存条件的变化可能会影响细胞因子水平的准确性.

研究的目的:

评估不同血液采集管和储存条件对细胞因子量化的影响.
评估修订的截止值对细胞因子测量临床相关性的影响.

主要方法:

血清和血中14种细胞因子水平的比较,使用四种类型的真空血液采集管.
使用AimPlex流细胞计量分析各种温度和持续时间存储的样本.
根据健康对照和临床影响评估重新计算截止值.

主要成果:

细胞因子水平在血中通常高于血清.
凝血清样本在4小时内离心,并在-20°C保存长达24小时,产生可靠的细胞因子值.
修订后的截止值显示,血症和严重肺炎患者的IL-6和IL-8的阳性率增加.

结论:

与血或凝块激活剂血清相比,细胞因子在凝血清中表现出更大的稳定性.
最佳量化需要在离心机和储存在-20°C后的4小时内或24小时内进行分析.
标准化预分析方法对于准确的细胞因子测量和改进的临床诊断至关重要.

关键词:

通过AimPlex的流动细胞计量.细胞因子细胞因子截止值是指截止值的截止值.传染病是一种传染性疾病.稳定的稳定性稳定的稳定性

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...