相关实验视频

Updated: May 1, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

纳米纤维素修饰的电极用于与微生物相关的口腔疾病生物标志物的同时生物感应

C Ramos-López¹, L García-Rodrigo¹, E Sánchez-Tirado¹

¹Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, 28040, Madrid, Spain.

Mikrochimica acta

|February 11, 2025

概括

一种新的双免疫传感器同时检测矩阵金属蛋白酶 (MMPs) MMP-9和MMP-13,这是口腔疾病的关键生物标志物. 这种传感器为临床诊断提供了一个快速而有竞争力的ELISA替代品.

科学领域:

生物医学工程生物医学工程
生物传感器是一种生物传感器.
分析化学分析化学

背景情况:

矩阵金属蛋白酶 (MMPs),特别是MMP-9和MMP-13,是与口腔疾病和其他炎症相关的关键生物标志物.
准确且同时检测这些生物标志物对于早期诊断和有效管理至关重要.
像ELISA这样的现有诊断方法可能耗时,可能无法同时检测.

研究的目的:

开发一种新型的双重免疫传感器,用于同时测定MMP-9和MMP-13.
使用印双碳电极,修改了减少的氧化石墨烯和晶体纳米纤维素,以提高固定和检测.
在临床样本 (如唾液和血清) 中验证免疫传感器的性能.

主要方法:

用减少的石墨烯氧化物和晶体纳米纤维素 (rGO/CNC) 修改的丝印双碳电极 (SPdCE) 的制造.
针对MMP-9和MMP-13的特定捕获抗体的固定,然后进行三明治类型的免疫试验.
使用基 (HQ) /H2O2系统进行度检测.

主要成果:

实现了0.25 ng mL-1的MMP-9和0.30 ng mL-1的MMP-13的低检测极限 (LOD).
从健康人群和牙周炎患者的唾液中成功确定了MMP-9和MMP-13水平.
从类风湿性关节炎 (RA) 和慢性阻塞性肺病 (COPD) 患者的血清样本中显示出有用性.
结果显示与商业ELISA套件有很强的一致性.

结论:

开发的rGO/CNC修改的SPdCE可使MMP-9和MMP-13的敏感和同时检测.
这种双重免疫传感器为诊断口腔和其他炎症疾病提供了快速 (<2小时) 和竞争力的ELISA替代方案.
该传感器显示出对点诊断和个性化医疗的巨大潜力.

关键词:

电压计是指对电压的测量.结晶的纳米纤维素.电化学免疫传感器电化学免疫传感器在MMP-13中,它是MMP-13.在MMP-9中,它是MMP-9的.矩阵金属蛋白酶是一种金属蛋白酶.口腔疾病是一种口腔疾病.减少的石墨烯氧化物.

更多相关视频

Fabrication of Fine Electrodes on the Tip of Hypodermic Needle Using Photoresist Spray Coating and Flexible Photomask for Biomedical Applications

Fabrication of Fine Electrodes on the Tip of Hypodermic Needle Using Photoresist Spray Coating and Flexible Photomask for Biomedical Applications

Published on: November 28, 2017

Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy

Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy

Published on: January 30, 2020

相关概念视频

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

The Oral Microbiota

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...