狗瘤透白细胞的转录特征的表征揭示了人类骨髓瘤的保存表达模式
Dylan T Ammons1, R Adam Harris2, Lyndah Chow3
1Flint Animal Cancer Center, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA. dylan.ammons@colostate.edu.
Cancer immunology, immunotherapy : CII
|February 11, 2025
概括
狗骨髓瘤中的瘤微环境 (TME) 改变了免疫细胞功能,促进了瘤的生长. 在OS TME内的免疫细胞显示抑制活动和增加疲劳标记,影响抗瘤反应.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 比较医学是一种比较医学.
背景情况:
- 免疫细胞在抗瘤反应中至关重要,但可以被瘤颠覆.
- 瘤微环境 (TME) 影响免疫细胞行为,促进瘤进展和免疫逃生.
研究的目的:
- 通过计算分析来自犬骨肉瘤 (OS) 的单细胞RNA测序 (scRNA-seq) 数据,以比较瘤透免疫细胞与循环白细胞.
- 研究OS TME如何影响透免疫细胞的比例和转录基因特征.
主要方法:
- 利用公开可用的scRNA-seq数据从狗的操作系统模型.
- 进行了差异性丰度和差异性基因表达分析.
- 与人类文献和人类scRNA-seq数据进行了对比.
主要成果:
- 在OS TME中观察到毛囊辅助T细胞,调节性T细胞和成熟的调节性树突细胞 (mregDCs) 的比例增加.
- 确定了T细胞耗尽标记物 (例如PDCD1) 和髓状细胞中免疫抑制分子的升级.
- 透的B细胞显示了蛋白质加工和内质网膜通路的丰富,表明激活.
结论:
- 狗的OS TME显著改变了透免疫细胞的转录程序,促进了免疫抑制的表型.
- 狗和人类之间存在对瘤透的保存转录组反应,具有显著的物种特异性差异.
- 这项研究为比较性免疫瘤学研究提供了宝贵的资源.
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Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
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