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编辑生长分化因子9基因通过非激活促进成熟因子来影响猪卵细胞在体外成熟

Yafei Jiao¹, Alian Liao¹, Xintong Jiang¹

¹State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, 510275, China.

|February 11, 2025

概括

编辑生长差异化因子9 (GDF9) 在体外成熟 (IVM) 过程中损害了猪卵细胞. 这种干扰会损害线粒体功能,并通过破坏关键信号通路来阻碍成熟,从而影响生殖性能.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
细胞生理学细胞生理学

背景情况:

生长分化因子9 (GDF9) 对卵细胞发育至关重要.
在猪卵细胞体外成熟 (IVM) 中GDF9的特定作用需要进一步阐明.

研究的目的:

为了研究GDF9在猪卵细胞IVM中的功能.
了解GDF9在卵细胞成熟中的作用背后的分子机制.

主要方法:

利用CRISPR-Cas9核糖蛋白复合体在生殖囊 (GV) 阶段在猪卵细胞中进行GDF9基因编辑.
在编辑后评估了卵细胞活力,线粒体功能,活性氧物种 (ROS) 水平和谷氨 (GSH) 含量.
分析了细胞内信号通路,包括cAMP-PKA,MOS,WEE1B/MYT1,CDC25B和CDK1激活.

主要成果:

在大约59%的培养卵细胞中实现了GDF9编辑,这导致了IVM期间的显著损伤.
编辑GDF9导致线粒体功能受损,ROS积累增加,GSH水平降低.
涉及cAMP-PKA,MOS,WEE1B/MYT1,CDC25B和CDK1的信号级联中断,失活的成熟促进因子 (MPF) 和抑制的第一个极体 (PB1) 挤出.

结论:

在猪卵细胞IVM中,GDF9起着关键的调节作用.
编辑GDF9通过cAMP-PKA信号通路干扰卵细胞成熟,影响生殖潜力.
这些发现为通过改进的IVM协议来提高猪的生殖性能提供了基础.

关键词:

在GDF9中,GDF9是GDF9的字体.基因编辑基因编辑猪卵细胞是猪的卵细胞.

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