茄子的转录因子 SmERF118 调节了光信号调节的安托西亚宁合成
Dalu Li1, Mengliang Zhu1, Shaohang Li1
1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Plant physiology and biochemistry : PPB
|February 11, 2025
概括
光诱导乙烯反应因子 (ERF) SmERF118 和 MYB 转录因子 SmMYB1 调节紫色茄子中的安托西亚宁合成. 这种 SmERF118-SmMYB1 模块在低光条件下增强了水果的颜色,提高了茄子的质量.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 农业科学 农业科学
背景情况:
- 安索亚宁合成对于紫色茄子 (Solanum melongena L.) 的质量至关重要,但低光条件对其产生负面影响.
- 调节茄子中因光引起的安东素积累的精确分子机制仍然不完全理解.
研究的目的:
- 鉴定和描述光诱导乙烯反应因子 (ERF) SmERF118在低光应激下调节茄子中安东氨酸合成中的作用.
- 阐明SmERF118控制氨酸生物合成的分子机制,包括它与其他调节因素的相互作用.
主要方法:
- 对SmERF118和SmMYB1.1.的基因克隆和表征.
- 对Smerf118过度表达的茄子进行转录组分析 (RNA-Seq).
- 酵母二杂交,双分子光补充,GST拉下,和共同免疫沉试验以确认蛋白质相互作用.
- 双露西法酶记者测定和短暂的过度表达实验,以评估基因激活.
主要成果:
- SmERF118充当了氨酸合成的积极调节剂,其表达是由bZIP转录因子SmHY5.5激活的.
- 过度表达SmERF118显著增加了氨酸积累和调高了类生物合成途径中的基因.
- SmERF118与MYB转录因子SmMYB1相互作用,形成一个复合物,共同激活关键的安托素生物合成基因 (SmCHS和SmDFR).
结论:
- 光信号通过SMERF118-SmMYB1调节模块促进茄子外皮中的安托西亚宁合成.
- 这项研究完善了对调节茄子色彩的分子网络的理解,并提供了对光介导的氨酸生物合成调节的见解.
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