通过雷利分离技术喷雾化LNP-mRNA的向性鼻腔输送
Hao-Ying Li1, Abhimata Paramanandana1, Sally Yunsun Kim1
1Institute of Pharmaceutical Sciences, King's College London, London SE1 9NH, United Kingdom.
International journal of pharmaceutics
|February 11, 2025
概括
这项研究引入了一种新的雷利分离原子化器,用于安全地通过鼻子输送脂质纳米粒子 (LNP) -mRNA疫苗. 该技术保持了LNP-mRNA的稳定性,并针对上呼吸道沉积,以进行有效的鼻腔疫苗接种.
科学领域:
- 生物技术是生物技术.
- 制药科学 制药科学
- 药物输送系统 药物输送系统
背景情况:
- 人们对mRNA疫苗的鼻腔输送越来越感兴趣.
- 脂质纳米粒子 (LNP) -mRNA复合体需要温和的气溶化技术来保持稳定性.
研究的目的:
- 开发和评估一种新的鼻腔原子化器,用于气溶LNP-mRNAs.
- 评估气溶液LNP-mRNAs的稳定性和沉积特征,用于鼻腔疫苗的输送.
主要方法:
- 使用了一种基于雷利分离原理的新型鼻腔原子化器.
- 分析了LNP-mRNAs在气溶化前后的生理化学特性.
- 使用阿尔伯塔理想化鼻腔入口 (AINI) 评估区域鼻腔沉积.
主要成果:
- 使用雷利分离技术的气溶剂保存了LNP-mRNA的生理化学特性.
- 生成的滴滴 (∼25微米) 具有高空气动力学直径 (>10微米),有利于上呼吸道沉积.
- 通过优化注射角度 (45°) 和空气流量 (7.5 L/分钟),在鼻轮机中实现了目标mRNA沉积 (>50-64%).
结论:
- 雷利分离技术提供了一种可行的方法,可以在不损害稳定性的情况下将LNP-mRNAs进行气溶剂.
- 这种方法促进了针对性的鼻腔疫苗接种,有可能提高疗效并减少全身暴露.
相关概念视频
01:22Drug Delivery: Miscellaneous Routes
Drug delivery methods like oral inhalation, nasal sprays, transdermal patches, eye drops, intravitreal injection, and rectal administration provide localized effects with reduced toxicity.
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs through the...
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs through the...
01:24Modified-Release Drug Delivery Systems: Site-Targeted
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.