Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

RBMX functional retrocopy safeguards brain development in a species-dependent context.

Brain : a journal of neurology·2026
Same author

Cdk7 promotes neuritogenesis in cortical neurons and contributes to social behavior in mice.

Cellular and molecular life sciences : CMLS·2026
Same author

Developmental gene expression patterns driving species-specific cortical features.

Nature·2026
Same author

Screening and Identification of TRPV1-Targeting Peptides from <i>Apis mellifera</i> Venom with Inflammatory Modulatory Potential.

Journal of natural products·2026
Same author

Adult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease.

Nature communications·2026
Same author

Assessing the Impact of Nutritional Stress on the Identification of Plastic-Associated Bacteria in Insect Gut Microbiota.

Microorganisms·2026

相关实验视频

Updated: May 28, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K

可消耗材料和缓冲组合的重大影响,用于低细胞数量的蛋白质样本制备.

Christopher Kune1, Sylvia Tielens2, Dominique Baiwir3

  • 1Mass Spectrometry Laboratory, MolSys Research Unit, University of Liege, Liege B-4000, Belgium.

Analytical chemistry
|February 11, 2025
PubMed
概括

蛋白质组学中的损失可以通过使用极性玻璃瓶材料和非离子洗剂来减少. 这些方法改善了蛋白质量化,并增加了已识别的,特别是对于像单细胞蛋白质组学这样的少量样本.

更多相关视频

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

3.8K
Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
10:21

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Published on: January 13, 2023

2.4K

相关实验视频

Last Updated: May 28, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

3.8K
Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
10:21

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Published on: January 13, 2023

2.4K

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 分析化学 分析化学
  • 生物化学 生物化学

背景情况:

  • 对聚烯瓶的吸附会导致大量损失,特别是对于疏水性.
  • 这种损失会影响蛋白质量化的准确性,特别是在小体积样本中.
  • 目前的方法缺乏在样本处理过程中减轻吸附的策略.

研究的目的:

  • 调查吸附到瓶表面及其对蛋白质学的影响.
  • 为了识别瓶子材料和缓冲剂添加剂,以尽量减少损失.
  • 提高蛋白质数据的质量和一致性,特别是对于低输入样本.

主要方法:

  • 对吸附的不同瓶装材料 (PP,PMMA,PET) 的评价.
  • 测试非离子洗剂 (PEO,DDM) 作为添加剂,以减少表面结合.
  • 使用无标签量化 (LFQ) 来量化信号和识别蛋白质.

主要成果:

  • 与PP相比,极性瓶子材料 (PMMA,PET) 显著减少了疏水性的损失.
  • 在优化的度下,非离子洗剂 (PEO,DDM) 提高了蛋白质的性能和一致性.
  • 建议导致低输入消化物的信号增加了10倍,并使单细胞蛋白质组学中的检测成为可能.

结论:

  • 使用极性小瓶材料和特定的非离子洗剂对于最大限度地减少蛋白质组学中的损失至关重要.
  • 这些策略大大提高了蛋白质组数据的质量,并使低输入样本的可靠分析成为可能.
  • 这些发现为增强蛋白质组学工作流程提供了实际解决方案,包括单细胞应用.