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可消耗材料和缓冲组合的重大影响,用于低细胞数量的蛋白质样本制备

Christopher Kune1, Sylvia Tielens2, Dominique Baiwir3

  • 1Mass Spectrometry Laboratory, MolSys Research Unit, University of Liege, Liege B-4000, Belgium.

Analytical chemistry
|February 11, 2025
PubMed
概括

蛋白质组学中的损失可以通过使用极性玻璃瓶材料和非离子洗剂来减少. 这些方法改善了蛋白质量化,并增加了已识别的,特别是对于像单细胞蛋白质组学这样的少量样本.

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相关概念视频

Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.