Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.2K
Heterochromatin02:38

Heterochromatin

9.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
9.9K
Abnormal Proliferation02:23

Abnormal Proliferation

4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Nucleosome Remodeling02:54

Nucleosome Remodeling

8.9K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
8.9K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Astrocytes exploit a neutrophil extracellular trap - like mechanism for myelin management: A new perspective on myelin-related disorders.

Brain, behavior, and immunity·2026
Same author

Use of novel synthetic analogs to decipher the interactome of phosphatidic acid species in neurosecretory cells.

Methods in enzymology·2026
Same author

Contribution of neuroepigenetics to HD - developmental and aging-related signatures.

Journal of Huntington's disease·2026
Same author

Group Lasso Based Selection for High-Dimensional Mediation Analysis.

Statistics in medicine·2026
Same author

Repeated restraint stress-induced increase in post-surgical somatosensory hypersensitivity and affective responding is mediated by β-adrenergic receptor activation and spinal NLRP3-IL1β signalling in male rats.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Thrombopoietin increases susceptibility for EVI1 + KMT2A-MLLT3-driven AML expressing stem cell genes linked to poor outcome.

Nature communications·2025

相关实验视频

Updated: May 28, 2025

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species
07:08

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Published on: February 27, 2018

9.5K

亨廷顿病中的加速表观遗传衰老涉及多镇压复合体1

Baptiste Brulé1,2,3, Rafael Alcalá-Vida1,2,3,4, Noémie Penaud1,2,3

  • 1Laboratoire de Neurosciences Cognitives et Adaptatives (LNCA), Strasbourg, France.

Nature communications
|February 11, 2025
PubMed
概括

亨廷顿症候群 亨廷顿症候群

更多相关视频

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

9.5K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.4K

相关实验视频

Last Updated: May 28, 2025

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species
07:08

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Published on: February 27, 2018

9.5K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

9.5K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.4K

科学领域:

  • 神经科学是一个神经科学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 遗传学 是一个遗传学.

背景情况:

  • 生理衰老导致表观遗传变化,损害细胞身份并抑制发育基因.
  • 亨廷顿病 (HD) 是一种神经退行性疾病,影响脆弱的神经元.
  • 表观遗传改变与神经退行性疾病的发病有关.

研究的目的:

  • 在亨廷顿病的小鼠模型中研究脆弱神经元的表观基因组景观.
  • 识别HD受影响神经元中加速衰老的潜在表观遗传机制.
  • 探索多抑制综合体 (PRC1和PRC2) 在疾病发病过程中的作用.

主要方法:

  • 细胞类型特定的多组体分析.
  • 在小鼠模型中,对三种疾病阶段进行时间分析.
  • 使用FANS-CUT&Tag进行表观基因组分析.
  • 已经研究过的质子修饰和多抑制复合体 (PRC) 活性.

主要成果:

  • 加快了HD纹状神经元中发育基因的减压.
  • 观察到质子重新乙化和H2AK119ub和H3K27me3标记的枯竭.
  • 确定了双价发育转录因子的PRC1依赖的活性化.
  • 证明了PRC1对应切换,改变了HD神经元中的异形表达.

结论:

  • 患HD的条状神经元表现出PRC1-依赖的加速表观遗传衰老.
  • 表观遗传失调,特别是涉及PRC1,有助于HD的发病.
  • 准PRC1可能为亨廷顿病提供治疗策略.