一种病原体效应器HaRxL10劫持了昼夜时钟组件CHE,以扰乱植物发育和免疫力
Mengyao Fu1,2, Yaoyu Zhou1,2, Xin Zhang1,2
1College of Life Sciences, Capital Normal University, Beijing, China.
Nature communications
|February 11, 2025
概括
植物病原体可以通过准昼夜时钟来操纵植物免疫系统. 这项研究揭示了一个oomycete效应器,HaRxL10,直接准和稳定CCA1徒步旅行 (CHE) 蛋白质,抑制植物免疫力.
科学领域:
- 植物生物学 植物生物学
- 分子植物病原体相互作用
- 循环节生物学 循环节生物学
背景情况:
- 植物的昼夜时钟是免疫系统的组成部分.
- 病原体可以破坏时钟基因表达.
- 病原体对时钟组件的影响机制在很大程度上是未知的.
研究的目的:
- 为了研究病原体如何影响植物生理时钟组件.
- 为了识别针对昼夜时钟的特定病原体效应因子.
- 阐明这种相互作用的分子机制.
主要方法:
- 酵母三混合测试检测蛋白质与蛋白质相互作用.
- 同免疫沉以确认蛋白质稳定.
- 定量实时PCR用于分析基因表达.
- 转录组测序以评估全球基因表达变化.
主要成果:
- 菌体效应器HaRxL10直接针对阿拉比多普西斯的中央时钟组件CCA1徒步旅行 (CHE).
- HaRxL10通过通过E3结合酶ZEITLUPE抑制其降解来稳定CHE.
- 累积的CHE抑制了自己的功能,抑制了植物的免疫力和生理过程.
结论:
- 这项研究确定了第一个植物病原体效应因子HaRxL10,该效应因子直接向植物昼夜钟组件 (CHE).
- HaRxL10操纵CHE的稳定性和功能,重编程植物转录组,抑制免疫力,改变宿主生理.
- 这项工作揭示了一种新的分子机制,病原体利用植物的昼夜时钟为自己的利益.
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