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相关概念视频

Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

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The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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ER Retrieval Pathway01:45

ER Retrieval Pathway

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In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
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Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

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Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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相关实验视频

Updated: May 28, 2025

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
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Rpl12 是一个保守的核糖体受体.

Yuting Chen1,2, Jiaxin Hu3, Pengwei Zhao1

  • 1Department of Biochemistry and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Nature cell biology
|February 11, 2025
PubMed
概括
此摘要是机器生成的。

Rpl12被确定为 ribofagy 的保留受体,这是 ribozome 周转的关键过程. 这一发现对细胞平衡至关重要,影响了饥饿,衰老和疾病模型.

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Study of Protein-protein Interactions in Autophagy Research
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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 自学研究 自学研究

背景情况:

  • 核糖是一种选择性的自过程,调节核糖体的周转.
  • 哺乳动物 рибо法基受体NUFIP1在酵母和线虫中缺乏同类物.

研究的目的:

  • 为了识别NUFIP1.1.以外的保护性 рибо法基受体.
  • 阐明Rpl12在不同生物体内的核糖和细胞平衡中的作用.

主要方法:

  • 研究了Rpl12-Atg8s的结合相互作用.
  • 分析了Atg1介导的Rpl12酸化及其在Atg11协会中的作用.
  • 利用Caenorhabditis elegans和Drosophila melanogaster的模型来研究核糖体缺陷.

主要成果:

  • Rpl12被确定为一种保存的核糖体受体.
  • 破坏Rpl12-Atg8s结合会导致核糖体蛋白和rRNA的积累.
  • Rpl12酸化增强了Atg11的结合,在饥饿期间触发了 ribofagy.
  • рибоophagy 缺乏加速细胞死亡,损害发育,缩短寿命,并导致运动缺陷.
  • 过度表达RPL12可以拯救的饥饿,衰老和Aβ诱导的运动缺陷.

结论:

  • Rpl12 作为保护的核糖体受体,对核糖体代谢至关重要.
  • 由Rpl12调解的 рибо法基对细胞平衡,生物体健康和寿命至关重要.
  • 对于与年龄有关的和神经退行性疾病,Rpl12是潜在的治疗点.