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通过生物信息学和机器学习方法识别临界生物标志物和在孕前的免疫透

Weiwen Li¹, Lijun Zhong¹, Kewen Zhao¹

¹Dongguan Maternal and Child Health Care Hospital, Dongguan, 523057, China.

BMC pregnancy and childbirth

|February 11, 2025

概括

孕前 (PE) 是一种妊娠并发症. 这项研究确定了HTRA4,PROCR和TXNIP作为与免疫细胞相关的关键基因,为早期PE诊断和治疗提供了潜在的生物标志物.

科学领域:

基因组学就是基因组学.
免疫学免疫学免疫学
生殖医学生殖医学

背景情况:

孕前 (PE) 是一种严重的怀孕障碍,其分子机制不清楚.
在PE中建议免疫系统参与胎盘功能和血管重塑.
识别关键基因和免疫细胞对于理解PE病理学至关重要.

研究的目的:

为了识别涉及到子宫前 (PE) 的关键基因.
调查这些基因与PE中的透免疫细胞之间的相关性.
发现早期诊断和治疗PE的潜在生物标志物.

主要方法:

利用了基因表达综合 (GEO) 数据集 (GSE74341,GSE160888) 用于差异表达分析,WGCNA和LASSO回归.
用于诊断评估的受体运行特征 (ROC) 分析和ssGSEA与免疫细胞相关联的基因.
在PE胎盘组织中使用实时RT-PCR验证了差异性基因表达.

主要成果:

在PE中确定了143个差异表达基因 (DEGs).
突出显示HTRA4,PROCR和TXNIP作为枢纽DEG和PE的潜在诊断标记.
证实HTRA4,PROCR,TXNIP和特定的免疫细胞 (马三角形T细胞,巨细胞,NK细胞,T毛囊辅助细胞) 之间存在密切的关系.
在PE胎盘组织中证实HTRA4,PROCR和TXNIP的差异表达.

结论:

已确定HTRA4,PROCR和TXNIP是孕前 (PE) 的潜在生物标志物.
这些基因为早期诊断和治疗PE提供了一种新的策略.
这些发现将特定的基因与免疫细胞透联系起来,进步对PE病变的理解.

关键词:

生物信息学是一种生物信息学.生物标志物生物标志物免疫细胞透透.孕前症孕前症

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